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顶部生物活性储存和溶解信息计算器

Anti-Phospho-BRAF (Thr401) Antibody (6W728)

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Anti-Phospho-BRAF (Thr401) Antibody (6W728)
产品编号 TMAC-03193
别名 p-BRAF (Thr401), p-BRAF (T401), BRAF (p-Thr401), BRAF (p-T401)

Anti-Phospho-BRAF (T401) Antibody (6W728) 是一种 Rabbit 抗体，靶向 Phospho-BRAF (T401)。Anti-Phospho-BRAF (T401) Antibody (6W728) 可用于 WB,ICC,IP。

Anti-Phospho-BRAF (Thr401) Antibody (6W728)

Anti-Phospho-BRAF (Thr401) Antibody (6W728)

Rating icon 还可以
Anti-Phospho-BRAF (Thr401) Antibody (6W728)
产品编号 TMAC-03193 别名 p-BRAF (Thr401), p-BRAF (T401), BRAF (p-Thr401), BRAF (p-T401)

Anti-Phospho-BRAF (T401) Antibody (6W728) 是一种 Rabbit 抗体，靶向 Phospho-BRAF (T401)。Anti-Phospho-BRAF (T401) Antibody (6W728) 可用于 WB,ICC,IP。

规格价格库存数量
50 μL
¥ 1,490
5日内发货
100 μL
¥ 2,490
5日内发货
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产品介绍

生物活性
产品描述
Anti-Phospho-BRAF (Thr401) Antibody (6W728) 是一种 Rabbit 抗体，靶向 Phospho-BRAF (Thr401)。Anti-Phospho-BRAF (Thr401) Antibody (6W728) 可用于 WB,ICC,IP。
别名p-BRAF (Thr401), p-BRAF (T401), BRAF (p-Thr401), BRAF (p-T401)
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of Phospho-B Raf (T401) on different lysates using anti-Phospho-B Raf (T401) antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse testis, Lane 2: PC-12.
2. ICC staining Phospho-B Raf (T401) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
3. ICC staining Phospho-B Raf (T401) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining Phospho-B Raf (T401) in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
应用
WB
ICC
IP
推荐剂量
WB: 1:1000-5000; ICC: 1:100-500
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted, with highest levels in the cerebrum and testis.
偶联与修饰
偶联
Unconjugated
其他可选修饰符
Phospho
抗原信息
免疫原
A synthesized phosphopeptide: human B Raf around the phosphorylation site of Thr401
抗原种属
human
Uniprot ID
P15056
化学信息
分子量Theoretical: 84 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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关键词

pBRAF (Thr401)pBRAF (T401)BRAF (pThr401)BRAF (pT401)BRAF (phospho-Thr401)BRAF (phospho-T401)

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4个月前
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