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Anti-Phospho-BRAF (Thr401) Antibody (2Z641)
还可以产品编号 TMAH-00884
别名 v-Raf murine sarcoma viral oncogene homolog B1, Serine/threonine-protein kinase B-raf, RAFB1, Proto-oncogene B-Raf, Phospho-BRAF (T401), p94, EC 2.7.11.1, BRAF1
Anti-Phospho-BRAF (Thr401) Antibody (2Z641) 是一种抗体，靶向 Phospho-BRAF (Thr401)。Anti-Phospho-BRAF (Thr401) Antibody (2Z641) 可用于 ELISA, WB, IF。
Anti-Phospho-BRAF (Thr401) Antibody (2Z641)
Anti-Phospho-BRAF (Thr401) Antibody (2Z641)
还可以产品编号 TMAH-00884 别名 v-Raf murine sarcoma viral oncogene homolog B1, Serine/threonine-protein kinase B-raf, RAFB1, Proto-oncogene B-Raf, Phospho-BRAF (T401), p94, EC 2.7.11.1, BRAF1
Anti-Phospho-BRAF (Thr401) Antibody (2Z641) 是一种抗体，靶向 Phospho-BRAF (Thr401)。Anti-Phospho-BRAF (Thr401) Antibody (2Z641) 可用于 ELISA, WB, IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,315 | 5日内发货 | |
| 100 μL | ¥ 2,190 | 5日内发货 |
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抗原信息
| 产品描述 | Anti-Phospho-BRAF (Thr401) Antibody (2Z641) is an antibody targeting Phospho-BRAF (Thr401). Anti-Phospho-BRAF (Thr401) Antibody (2Z641) can be used in ELISA, WB, IF. |
| 别名 | v-Raf murine sarcoma viral oncogene homolog B1, Serine/threonine-protein kinase B-raf, RAFB1, Proto-oncogene B-Raf, Phospho-BRAF (T401), p94, EC 2.7.11.1, BRAF1 |
| Ig Type | Rabbit IgG |
| 克隆号 | 2Z641 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in A549 whole cell lysate(treated with EGF or not) -All lanes Phospho-BRAF antibody at 1.3μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 85 KDa -Observed band size: 85 KDa 2. Immunofluorescence staining of HepG2 cells with TMAH-00884 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| 应用 | ELISA, WB, IF |
| 推荐剂量 | WB:1:500-1:5000; IF:1:20-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Nucleus. Cytoplasm. Cell membrane. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway. May play a role in the postsynaptic responses of hippocampal neurons. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human Phospho-BRAF (T401) |
| 抗原种属 | Human |
| 基因ID | 673 |
| Uniprot ID | |
| 研究领域 | Cell Biology |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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