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Anti-Phospho-AQP2 (Ser256) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-AQP2 (Ser256)。Anti-Phospho-AQP2 (Ser256) Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-Phospho-AQP2 (Ser256) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-AQP2 (Ser256)。Anti-Phospho-AQP2 (Ser256) Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,165 | 5日内发货 | |
100 μL | ¥ 1,960 | 5日内发货 | |
200 μL | ¥ 2,780 | 5日内发货 |
产品描述 | Anti-Phospho-AQP2 (Ser256) Polyclonal Antibody is a Rabbit antibody targeting Phospho-AQP2 (Ser256). Anti-Phospho-AQP2 (Ser256) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
交叉反应 | Human,Dog (predicted:Mouse,Rat,Chicken,Pig,Cow,Horse,Rabbit,Monkey) |
验证活性 | 1. Sample: MDCk (Dog) Cell Lysate at 30 μg Primary: Anti-phospho-AQP2 (Ser256) (TMAB-01391) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa 2. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-AQP2 (Ser256)) Polyclonal Antibody, Unconjugated (TMAB-01391) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Apical cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Note=Shuttles from vesicles to the apical membrane. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | This gene encodes a water channel protein located in the kidney collecting tubule. It belongs to the MIP/aquaporin family, some members of which are clustered together on chromosome 12q13. Mutations in this gene have been linked to autosomal dominant, and recessive forms of nephrogenic diabetes insipidus. Belongs to the MIP/aquaporin (TC 1.A.8) family. |
免疫原 | KLH conjugated synthesised phosphopeptide: human AQP2 around the phosphorylation site of Ser256 |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Channels |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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