Anti-Phospho-AQP2 (Ser256) Polyclonal Antibody is a Rabbit antibody targeting Phospho-AQP2 (Ser256). Anti-Phospho-AQP2 (Ser256) Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human,Dog (predicted:Mouse,Rat,Chicken,Pig,Cow,Horse,Rabbit,Monkey)

1. Sample:
MDCk (Dog) Cell Lysate at 30 μg
Primary: Anti-phospho-AQP2 (Ser256) (TMAB-01391) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
2. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-AQP2 (Ser256)) Polyclonal Antibody, Unconjugated (TMAB-01391) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthesised phosphopeptide: human AQP2 around the phosphorylation site of Ser256

Human

AQP2

Aquaporin-2

Channels

Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.