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Anti-Phospho-4EBP1 (Thr37) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-4EBP1 (Thr37)。Anti-Phospho-4EBP1 (Thr37) Polyclonal Antibody 可用于 WB,IHC。
Anti-Phospho-4EBP1 (Thr37) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-4EBP1 (Thr37)。Anti-Phospho-4EBP1 (Thr37) Polyclonal Antibody 可用于 WB,IHC。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,480 | 5日内发货 | |
100 μL | ¥ 2,240 | 5日内发货 |
产品描述 | Anti-Phospho-4EBP1 (Thr37) Polyclonal Antibody is a Rabbit antibody targeting Phospho-4EBP1 (Thr37). Anti-Phospho-4EBP1 (Thr37) Polyclonal Antibody can be used in WB,IHC. |
别名 | p-4EBP1 (Thr37), p-4EBP1 (T37), 4EBP1 (p-Thr37), 4EBP1 (p-T37) |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of extracts from MDA-MB-435 cells, untreated or EGF-treated (200 ng/ml, 30min), using 4E-BP1 (Ab-37) antibody (#21215, Lane 1 and 2) and 4E-BP1 (phospho-Thr37) antibody (TMAC-00021, Lane 3 and 4). 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using 4E-BP1 (phospho-Thr36) antibody (TMAC-00021). |
应用 | |
推荐剂量 | WB: 1:500-1000; IHC: 1:50-100 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
构建方式 | Polyclonal Antibody |
纯化方式 | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
性状 | Liquid |
缓冲液 | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. |
偶联 | Unconjugated |
其他可选修饰符 | Phospho |
免疫原 | Peptide sequence around phosphorylation site of threonine 37(S-T-T(p)-P-G) derived from Human 4E-BP1 |
抗原种属 | human |
Uniprot ID |
分子量 | Actual: 18 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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