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Anti-PDPK1 Antibody (6S392)
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产品编号 TMAC-03143
别名 Protein kinase, PRO0461, PkB like 1, PkB kinase like gene 1, PkB kinase, PDPK2P, PDPK1_HUMAN, Pdpk1, PDPK 2, PDK1, OTTHUMP00000174525, OTTHUMP00000159110, OTTHUMP00000159109, MGC35290, MGC20087, hPDK1, hPDK 1, 3-phosphoinositide-dependent protein kinase 1, 3 phosphoinositide dependent protein kinase 1
Anti-PDPK1 Antibody (6S392) 是一种 Rabbit 抗体，靶向 PDPK1。Anti-PDPK1 Antibody (6S392) 可用于 WB,ICC/IF,IHC。
Anti-PDPK1 Antibody (6S392)
Anti-PDPK1 Antibody (6S392)
还可以产品编号 TMAC-03143 别名 Protein kinase, PRO0461, PkB like 1, PkB kinase like gene 1, PkB kinase, PDPK2P, PDPK1_HUMAN, Pdpk1, PDPK 2, PDK1, OTTHUMP00000174525, OTTHUMP00000159110, OTTHUMP00000159109, MGC35290, MGC20087, hPDK1, hPDK 1, 3-phosphoinositide-dependent protein kinase 1, 3 phosphoinositide dependent protein kinase 1
Anti-PDPK1 Antibody (6S392) 是一种 Rabbit 抗体，靶向 PDPK1。Anti-PDPK1 Antibody (6S392) 可用于 WB,ICC/IF,IHC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,480 | 5日内发货 | |
| 100 μL | ¥ 2,495 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-PDPK1 Antibody (6S392) is a Rabbit antibody targeting PDPK1. Anti-PDPK1 Antibody (6S392) can be used in WB,ICC/IF,IHC. |
| 别名 | Protein kinase, PRO0461, PkB like 1, PkB kinase like gene 1, PkB kinase, PDPK2P, PDPK1_HUMAN, Pdpk1, PDPK 2, PDK1, OTTHUMP00000174525, OTTHUMP00000159110, OTTHUMP00000159109, MGC35290, MGC20087, hPDK1, hPDK 1, 3-phosphoinositide-dependent protein kinase 1, 3 phosphoinositide dependent protein kinase 1 |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of PDPK1 on different lysates using anti-PDPK1 antibody at 1/1,000 dilution. Positive control: Lane 1: 293T, Lane 2: PC-12, Lane 3: HL-60. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PDPK1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PDPK1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PDPK1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PDPK1 antibody. Counter stained with hematoxylin. 6. ICC staining PDPK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining PDPK1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining PDPK1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:100-500 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | PDPK1 (3-phosphoinositide dependent protein kinase 1), also known as PDK1, PDPK2, PDPK2P or PRO0461, is 556 amino acid ubiquitously expressed protein that localizes to the cell membrane, cytoplasm and nucleus. Acting as a master kinase, PDPK1 phosphorylates and activates a subgroup of the AGC family of protein kinases. PDPK1 is involved in mediating signal transduction for controlling proliferation, survival, and growth of developing pancreatic beta cells, regulating Ca2+ uptake and Ca2+-activated K+ channels of mast cells, regulation of chemotaxis and motility of vascular endothelial cells, cardiac homeostasis, and thymocyte development. Belonging to the protein kinase superfamily, PDPK1 contains a PH domain, which play an essential role in homodimerization, localization and nuclear import of PDPK1, and a protein kinase domain. PDPK1 exists as five alternatively spliced isoforms and is encoded by a gene located on human chromosome 16p13.3. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 63/48 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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