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顶部生物活性储存和溶解信息计算器

Anti-PDHA1 Antibody (9A259)

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Anti-PDHA1 Antibody (9A259)
产品编号 TMAY-02750

Anti-PDHA1 Antibody (9A259) 是一种 Rabbit 抗体，靶向 PDHA1。Anti-PDHA1 Antibody (9A259) 可用于 ICC/IF。

Anti-PDHA1 Antibody (9A259)
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Anti-PDHA1 Antibody (9A259)

Rating icon 还可以
Anti-PDHA1 Antibody (9A259)
产品编号 TMAY-02750

Anti-PDHA1 Antibody (9A259) 是一种 Rabbit 抗体，靶向 PDHA1。Anti-PDHA1 Antibody (9A259) 可用于 ICC/IF。

规格价格库存数量
100 μL
¥ 1,684
5日内发货
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产品介绍

生物活性
产品描述
Anti-PDHA1 Antibody (9A259) is a Rabbit antibody targeting PDHA1. Anti-PDHA1 Antibody (9A259) can be used in ICC/IF.
Ig Type
Rabbit IgG
克隆号
9A259
交叉反应
Human
验证活性
Immunofluorescence staining of PDHA1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PDHA1 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
应用
ICC/IF
推荐剂量
ICC-IF: 1:20-1:100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human PDHA1 (rh PDHA1; P08559-1Phe30-Ser390).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景PDHA1, also known as C54G1, is an alpha subunit of pyruvate dehydrogenase. Pyruvate dehydrogenase, together with dihydrolipoamide acetyltransferase and lipoamide dehydrogenase, composes the pyruvate dehydrogenase (PDH) complex. The PDH complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. PDHA1 plays a key role in the function of the PDH complex. Defects in PDHA1 can cause pyruvate dehydrogenase E1-alpha deficiency. Defects in PDHA1 also are the cause of X-linked Leigh syndrome (X-LS). X-LS is an early-onset progressive neurodegenerative disorder with a characteristic neuropathology consisting of focal, bilateral lesions in one or more areas of the central nervous system, including the brainstem, thalamus, basal ganglia, cerebellum, and spinal cord.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: Human PDHA1 Protein
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

计算器

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关键词

somatic formPyruvate dehydrogenase E1 component subunit α, somatic form, mitochondrialPyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrialPyruvate dehydrogenase E1 component subunit a, somatic form, mitochondrialPHE1APDHE1A type-IPDHE1-A type IPDHA1PDHA 1mitochondrial

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