- 全部删除
- 您的购物车当前为空
Anti-PBK/TOPK Antibody (6C84) 是一种 Mouse 抗体,靶向 PBK/TOPK。Anti-PBK/TOPK Antibody (6C84) 可用于 WB,ICC,IHC,FCM。
Anti-PBK/TOPK Antibody (6C84) 是一种 Mouse 抗体,靶向 PBK/TOPK。Anti-PBK/TOPK Antibody (6C84) 可用于 WB,ICC,IHC,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,985 | 5日内发货 | |
100 μL | ¥ 2,980 | 5日内发货 |
产品描述 | Anti-PBK/TOPK Antibody (6C84) is a Mouse antibody targeting PBK/TOPK. Anti-PBK/TOPK Antibody (6C84) can be used in WB,ICC,IHC,FCM. |
别名 | TOPK, SPK, PDZ binding kinase, Nori-3, HEL164, CT84 |
交叉反应 | Human |
验证活性 | 1. Western blot analysis of PBK on human PBK recombinant protein using anti-PBK antibody at 1/1,000 dilution. 2. Western blot analysis of PBK on A431 cell lysate using anti-PBK antibody at 1/1,000 dilution. 3. Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-PBK antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PBK antibody. Counter stained with hematoxylin. 5. ICC staining PBK (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of Hela cells with PBK antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |
应用 | |
推荐剂量 | WB: 1:500; IHC: 1:100-500; ICC: 1:100-500; FCM: 1:100-200 |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
构建方式 | Hybridoma Monoclonal Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
研究背景 | Protein kinases comprise a large group of encoded factors that regulate cellular processes by catalyzing the transfer of a phosphate group to a hydroxyl acceptor in serine, threonine or tyrosine residues. Kinases are capable of influencing the oncogenic potential of cell sytems at the level of oncoprotein or tumor suppressor protein phosphorylation states. Human PDZ-binding kinase, known as PBK, is a 322 amino acid, T/SXV motif-containing serine/threonine kinase that is abuntant in placenta and absent from adult brain tissue. A PDZ domain in the tumor suppressor protein Dlg can coordinate with the T/SXV motif of PBK. The cell cycle checkpoint kinase Cdc2/cyclin B is an upstream effector of PBK that can phosphorylate and activate PBK. Active PBK may associate with PDZ-containing proteins and influence cell cycle control or cellular proliferation. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 36 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
评论内容