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Anti-PBK/TOPK Antibody (6C84)

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Anti-PBK/TOPK Antibody (6C84)
产品编号 TMAC-03090
别名 TOPK, SPK, PDZ binding kinase, Nori-3, HEL164, CT84

Anti-PBK/TOPK Antibody (6C84) 是一种 Mouse 抗体,靶向 PBK/TOPK。Anti-PBK/TOPK Antibody (6C84) 可用于 WB,ICC,IHC,FCM。

Anti-PBK/TOPK Antibody (6C84)

Anti-PBK/TOPK Antibody (6C84)

Rating icon 还可以
Anti-PBK/TOPK Antibody (6C84)
产品编号 TMAC-03090 别名 TOPK, SPK, PDZ binding kinase, Nori-3, HEL164, CT84

Anti-PBK/TOPK Antibody (6C84) 是一种 Mouse 抗体,靶向 PBK/TOPK。Anti-PBK/TOPK Antibody (6C84) 可用于 WB,ICC,IHC,FCM。

规格价格库存数量
50 μL
¥ 1,985
5日内发货
100 μL
¥ 2,980
5日内发货
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产品介绍

生物活性
产品描述
Anti-PBK/TOPK Antibody (6C84) is a Mouse antibody targeting PBK/TOPK. Anti-PBK/TOPK Antibody (6C84) can be used in WB,ICC,IHC,FCM.
别名TOPK, SPK, PDZ binding kinase, Nori-3, HEL164, CT84
交叉反应
Human
验证活性
1. Western blot analysis of PBK on human PBK recombinant protein using anti-PBK antibody at 1/1,000 dilution.
2. Western blot analysis of PBK on A431 cell lysate using anti-PBK antibody at 1/1,000 dilution.
3. Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-PBK antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PBK antibody. Counter stained with hematoxylin.
5. ICC staining PBK (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. Flow cytometric analysis of Hela cells with PBK antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
应用
推荐剂量
WB: 1:500; IHC: 1:100-500; ICC: 1:100-500; FCM: 1:100-200
抗体种类
Monoclonal
宿主来源Mouse
构建方式Hybridoma Monoclonal Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
研究背景Protein kinases comprise a large group of encoded factors that regulate cellular processes by catalyzing the transfer of a phosphate group to a hydroxyl acceptor in serine, threonine or tyrosine residues. Kinases are capable of influencing the oncogenic potential of cell sytems at the level of oncoprotein or tumor suppressor protein phosphorylation states. Human PDZ-binding kinase, known as PBK, is a 322 amino acid, T/SXV motif-containing serine/threonine kinase that is abuntant in placenta and absent from adult brain tissue. A PDZ domain in the tumor suppressor protein Dlg can coordinate with the T/SXV motif of PBK. The cell cycle checkpoint kinase Cdc2/cyclin B is an upstream effector of PBK that can phosphorylate and activate PBK. Active PBK may associate with PDZ-containing proteins and influence cell cycle control or cellular proliferation.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
化学信息
分子量Theoretical: 36 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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4个月前
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