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Anti-Osteopontin Antibody (5D926)
还可以产品编号 TMAH-01129
别名 secreted phosphoprotein 1, OPN, ETA-1, BSPI, BNSP
Anti-Osteopontin Antibody (5D926) 是一种抗体，靶向 Osteopontin。Anti-Osteopontin Antibody (5D926) 可用于 ELISA, WB, IHC。
Anti-Osteopontin Antibody (5D926)
Anti-Osteopontin Antibody (5D926)
还可以产品编号 TMAH-01129 别名 secreted phosphoprotein 1, OPN, ETA-1, BSPI, BNSP
Anti-Osteopontin Antibody (5D926) 是一种抗体，靶向 Osteopontin。Anti-Osteopontin Antibody (5D926) 可用于 ELISA, WB, IHC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,305 | 5日内发货 | |
| 100 μL | ¥ 2,195 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-Osteopontin Antibody (5D926) is an antibody targeting Osteopontin. Anti-Osteopontin Antibody (5D926) can be used in ELISA, WB, IHC. |
| 别名 | secreted phosphoprotein 1, OPN, ETA-1, BSPI, BNSP |
| Ig Type | Rabbit IgG |
| 克隆号 | 5D926 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in: 293T whole cell lysate, U-251 whole cell lysate, U-87 whole cell lysate -All lanes: Osteopontin Antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 36, 34, 33, 34, 34 kDa -Observed band size: 36 kDa 2. IHC image of TMAH-01129 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| 应用 | ELISA, WB, IHC |
| 推荐剂量 | WB:1:500-1:5000; IHC:1:50-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Secreted. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human Osteopontin |
| 抗原种属 | Human |
| 基因ID | 6696 |
| Uniprot ID | |
| 研究领域 | Cancer, Cardiovascular, Signal transduction, Stem cells |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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