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Anti-NT5C3A Antibody (5M344)
还可以产品编号 TMAY-02656
别名 UMPH1, UMPH, PSN1, POMP, PN-I, P5N-1, P5'N-1, P5N1, p36, NT5C3, hUMP1, HSPC233, cN-III
Anti-NT5C3A Antibody (5M344) 是一种 Rabbit 抗体，靶向 NT5C3A。Anti-NT5C3A Antibody (5M344) 可用于 WB,IP。
Anti-NT5C3A Antibody (5M344)
Anti-NT5C3A Antibody (5M344)
还可以产品编号 TMAY-02656 别名 UMPH1, UMPH, PSN1, POMP, PN-I, P5N-1, P5'N-1, P5N1, p36, NT5C3, hUMP1, HSPC233, cN-III
Anti-NT5C3A Antibody (5M344) 是一种 Rabbit 抗体，靶向 NT5C3A。Anti-NT5C3A Antibody (5M344) 可用于 WB,IP。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 100 μL | ¥ 2,289 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-NT5C3A Antibody (5M344) is a Rabbit antibody targeting NT5C3A. Anti-NT5C3A Antibody (5M344) can be used in WB,IP. |
| 别名 | UMPH1, UMPH, PSN1, POMP, PN-I, P5N-1, P5'N-1, P5N1, p36, NT5C3, hUMP1, HSPC233, cN-III |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 5M344 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-NT5C3 rabbit monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:38 kDa. -Observed band size:39 kDa. 2. NT5C3 was immunoprecipitated using: -Lane A:0.5 mg 293T Whole Cell Lysate. -Lane B:0.5 mg Jurkat Whole Cell Lysate -0.5 µL anti-NT5C3 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-NT5C3 rabbit monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 38 kDa. -Observed band size: 38 kDa |
| 应用 | WB,IP |
| 推荐剂量 | WB: 1:500-1:1000; IP: 1-2 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human NT5C3A / UMPH1 (rh NT5C3A / UMPH1; NP_001002010; Met12-Leu297). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | NT5C3A (5'-Nucleotidase, Cytosolic IIIA) is a Protein Coding gene. This gene encodes a member of the 5'-nucleotidase family of enzymes that catalyze the dephosphorylation of nucleoside 5'-monophosphates. The encoded protein is the type 1 isozyme of pyrimidine 5' nucleotidase and catalyzes the dephosphorylation of pyrimidine 5' monophosphates. NT5C3A expression required both an intronic IFN-stimulated response element and the IFN-stimulated transcription factor IRF1. Overexpression of NT5C3A, but not of its catalytic mutants, suppressed IL-8 production by HEK293 cells. NT5C3A-stimulated sirtuin activity resulted in deacetylation of histone H3 and the NF-kappaB subunit RelA (also known as p65), both of which were associated with the proximal region of the Il8 promoter, thus repressing the transcription of Il8 Together. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human NT5C3A / UMPH1 Protein |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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