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Anti-NONO Antibody (8D270) 是一种 Rabbit 抗体,靶向 NONO。Anti-NONO Antibody (8D270) 可用于 WB,ICC,IF,IHC。
Anti-NONO Antibody (8D270) 是一种 Rabbit 抗体,靶向 NONO。Anti-NONO Antibody (8D270) 可用于 WB,ICC,IF,IHC。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,495 | 5日内发货 | |
100 μL | ¥ 2,480 | 5日内发货 |
产品描述 | Anti-NONO Antibody (8D270) is a Rabbit antibody targeting NONO. Anti-NONO Antibody (8D270) can be used in WB,ICC,IF,IHC. |
别名 | p54nrb, NRB54, Non-POU domain-containing octamer-binding protein, NonO protein, NMT55, DNA-binding p52/p100 complex, 55 kDa nuclear protein, 54 kDa nuclear RNA- and DNA-binding protein, 52 kDa subunit |
Ig Type | IgG |
克隆号 | 8D270 |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of p54nrb on 293 (1) and SH-SY-5Y (2) cell lysate using anti-p54nrb antibody at 1/500 dilution. 2. Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti- p54nrb antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- p54nrb antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti- p54nrb antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-p54nrb antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- p54nrb antibody. Counter stained with hematoxylin. 7. ICC staining p54nrb in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Found in both primary and transformed human cells, paraspeckles are discrete bodies in the interchromatin nucleoplasmic space which contain p54/nrb (nuclear RNA-binding protein) and at least two other RNA-binding proteins, paraspeckle protein 1 (PSP1) and paraspeckle protein 2 (PSP2). Paraspeckles often co-localize with splicing speckles, which are the site of splicing factor accumulation. Paraspeckle proteins, including p54/nrb, move dynamically between the nucleolus and paraspeckles and translocate to distinct caps in the nucleolar periphery when transcription is inhibited. Originally purified from HeLa cells, the nuclear p54/nrb has two RNA recognition motifs and shares extensive homology with both the human splicing factor PSF and Drosophila NONA/BJ6, which is required for normal vision and courtship. The shared domain between these proteins is termed a DBHS (Drosophila behavior, human splicing) domain and may play a role in regulating various pathways at the level of pre-mRNA splicing. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein: C-terminal human p54nrb |
抗原种属 | human |
Uniprot ID |
分子量 | Theoretical: 54 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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