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Anti-Neprilysin/CD10 Antibody (5M454) 是一种 Rabbit 抗体,靶向 Neprilysin/CD10。Anti-Neprilysin/CD10 Antibody (5M454) 可用于 WB,FCM,IHC,ICC。
Anti-Neprilysin/CD10 Antibody (5M454) 是一种 Rabbit 抗体,靶向 Neprilysin/CD10。Anti-Neprilysin/CD10 Antibody (5M454) 可用于 WB,FCM,IHC,ICC。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,495 | 5日内发货 | |
100 μL | ¥ 2,485 | 5日内发货 |
产品描述 | Anti-Neprilysin/CD10 Antibody (5M454) is a Rabbit antibody targeting Neprilysin/CD10. Anti-Neprilysin/CD10 Antibody (5M454) can be used in WB,FCM,IHC,ICC. |
别名 | SFE, SCA43, NEP, EPN, CMT2T, CD10, CALLA |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD10 antibody. Counter stained with hematoxylin. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CD10 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CD10 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD10 antibody. Counter stained with hematoxylin. 5. ICC staining CD10 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining CD10 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of Jurkat cells with CD10 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:500-1000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | CD10, also called the common acute lymphoblastic leukemia antigen (CALLA) and neutral endopeptidase (NEP), is a type II integral membrane glycoprotein. CD10 acts as a zinc metalloprotease that cleaves a variety of biologically active peptides including Angiotensins I and II. It is expressed on early B and T lymphoid precursors, B blasts, some granulocytes, bone marrow stromal cells and certain epithelial cells including some tumor cell lines. CD10 is used as a marker of common acute lymphocytic leukemias and some lymphomas. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 100 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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