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Anti-NDUFS3 Antibody (2K991) 是一种抗体,靶向 NDUFS3。Anti-NDUFS3 Antibody (2K991) 可用于 ELISA, WB, IHC。
Anti-NDUFS3 Antibody (2K991) 是一种抗体,靶向 NDUFS3。Anti-NDUFS3 Antibody (2K991) 可用于 ELISA, WB, IHC。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,315 | 5日内发货 | |
100 μL | ¥ 2,185 | 5日内发货 |
产品描述 | Anti-NDUFS3 Antibody (2K991) is an antibody targeting NDUFS3. Anti-NDUFS3 Antibody (2K991) can be used in ELISA, WB, IHC. |
别名 | NDUFS 3, NADH-ubiquinone oxidoreductase 30 kDa subunit, NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial, EC 1.6.99.3, EC 1.6.5.3, Complex I-30kD, CI-30kD |
Ig Type | Rabbit IgG |
克隆号 | 2K991 |
交叉反应 | Human |
验证活性 | 1. Western Blot -Positive WB detected in: 293 whole cell lysate, HepG2 whole cell lysate, -All lanes: NDUFS3 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 31, 15 KDa -Observed band size: 31 kDa 2. IHC image of TMAH-00807 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
应用 | ELISA, WB, IHC |
推荐剂量 | WB:1:500-1:5000; IHC:1:50-1:200. |
抗体种类 | Monoclonal |
亚细胞定位 | Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. |
构建方式 | Recombinant Antibody |
纯化方式 | Affinity-chromatography |
性状 | Liquid |
缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I. |
偶联 | Unconjugated |
免疫原 | A synthetic peptide: Human NDUFS3 |
抗原种属 | Human |
基因ID | 4722 |
Uniprot ID | |
研究领域 | Cancer, Cell biology, Metabolism, Signal transduction |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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