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Anti-NAA10/ARD1 Antibody (8M884)
还可以产品编号 TMAY-02716
Anti-NAA10/ARD1 Antibody (8M884) 是一种 Mouse 抗体，靶向 NAA10/ARD1。Anti-NAA10/ARD1 Antibody (8M884) 可用于 WB,IP。
Anti-NAA10/ARD1 Antibody (8M884)
Anti-NAA10/ARD1 Antibody (8M884)
还可以产品编号 TMAY-02716
Anti-NAA10/ARD1 Antibody (8M884) 是一种 Mouse 抗体，靶向 NAA10/ARD1。Anti-NAA10/ARD1 Antibody (8M884) 可用于 WB,IP。
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抗原信息
| 产品描述 | Anti-NAA10/ARD1 Antibody (8M884) is a Mouse antibody targeting NAA10/ARD1. Anti-NAA10/ARD1 Antibody (8M884) can be used in WB,IP. |
| Ig Type | Monoclonal Mouse IgG2b |
| 克隆号 | 8M884 |
| 交叉反应 | Human |
| 验证活性 | 1. ARD1A was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate -0.5 µL anti-ARD1A mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-ARD1A mouse monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 33 kDa. -Observed band size: 26 kDa. 2. Anti-ARD1A mouse monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:26 kDa. -Observed band size:33 kDa |
| 应用 | WB,IP |
| 推荐剂量 | WB: 1:500-1:1000; IP: 0.2-1 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ARD1 (rh ARD1; TMPY-03339; P41227; Met1-Ser235). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | ARD1 is a member of the 2-kDa ARF protein family. It is a multifunctional protein. ARD1 has an 18-kDa ADP-ribosylation factor (ARF) domain at the C-terminus (amino acids 43-574), and a 46-kDa N-terminal domain (amino acids 1-42). The C-terminal region of ARD1 may be involved in the formation of both ARD1-ARD1 and ARD1-NAT1 complexes. ARD1 and NAT1 genes are required for the expression of an N-terminal protein acetyltransferase. This activity is required for full repression of the silent mating-type locus HML, for sporulation, and for entry into G. Recombinant ARD1 (amino acids 1-574) or its RING finger domain (amino acids 1-11) produced polyubiquitylated proteins when incubated in vitro with a mammalian E1, an E2 enzyme, ATP, and ubiquitin. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human ARD1 protein (TMPY-03339) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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