Anti-MYH1 Polyclonal Antibody is a Rabbit antibody targeting MYH1. Anti-MYH1 Polyclonal Antibody can be used in IHC-P,WB,IHC-Fr,IF.

IgG

Rat,Mouse,Human

1. Paraformaldehyde-fixed, paraffin embedded (mouse tongue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (MYH1) Polyclonal Antibody, Unconjugated (TMAB-09149) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat tongue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (MYH1) Polyclonal Antibody, Unconjugated (TMAB-09149) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (MYH1) Polyclonal Antibody, Unconjugated (TMAB-09149) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
4. Sample:
Lane 1: Recombinant human MYH1 protein, N-His
Primary: Anti-MYH1 (TMAB-09149) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: kDa
Observed band size: 44 kDa
5. Negative sample: Paraformaldehyde-fixed, paraffin embedded (Mouse liver);
Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min;
Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (MYH1) Polyclonal Antibody, Unconjugated (TMAB-09149) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
6. Negative sample: Paraformaldehyde-fixed, paraffin embedded (rat liver);Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (MYH1) Polyclonal Antibody, Unconjugated (TMAB-09149) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (Mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (MYH1) Polyclonal Antibody, Unconjugated (TMAB-09149) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
8. Negative sample: Paraformaldehyde-fixed, paraffin embedded (human brain);Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (MYH1) Polyclonal Antibody, Unconjugated (TMAB-09149) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Rat Tongue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MYH1 Polyclonal Antibody, Unconjugated (TMAB-09149) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.
10. Paraformaldehyde-fixed, paraffin embedded Mouse Tongue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MYH1 Polyclonal Antibody, Unconjugated (TMAB-09149) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.
11. Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MYH1 Polyclonal Antibody, Unconjugated (TMAB-09149) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.

IHC-P: 1:100-500; WB: 1:500-2000; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

Recombinant Protein: human MYH1 protein

Human

MYH1

Myosin-1

Muscle contraction.