Anti-MRC1 Antibody (6S133) is a Rabbit antibody targeting MRC1. Anti-MRC1 Antibody (6S133) can be used in IHC-P,IHC-Fr,ICC/IF,IF.

IgG

6S133

Human

1. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD206) Monoclonal Antibody, Unconjugated (TMAB-01165) at 1:300 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD206) Monoclonal Antibody, Unconjugated (TMAB-01165) at 1:300 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD206) Monoclonal Antibody, Unconjugated (TMAB-01165) at 1:300 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD206) Monoclonal Antibody, Unconjugated (TMAB-01165) at 1:300 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD206 Monoclonal Antibody, Unconjugated (TMAB-01165) at 1:1000 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD206 Monoclonal Antibody, Unconjugated (TMAB-01165) at 1:1000 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD206 Monoclonal Antibody, Unconjugated (TMAB-01165) at 1:1000 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
8. 4% Paraformaldehyde-fixed Molt-4 (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (MRC1) monoclonal Antibody, unconjugated (TMAB-01165) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control.

IHC-P: 1:200-1000; IHC-Fr: 1:200-1000; ICC/IF: 1:50-200; IF: 1:200-1000

Monoclonal

KLH conjugated synthetic peptide: human CD206

Human

Receptors,Macrophage / Inflamm.