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Anti-MMBP Antibody (5B968) 是一种 Rabbit 抗体,靶向 MMBP。Anti-MMBP Antibody (5B968) 可用于 WB。
Anti-MMBP Antibody (5B968) 是一种 Rabbit 抗体,靶向 MMBP。Anti-MMBP Antibody (5B968) 可用于 WB。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,480 | 5日内发货 | |
100 μL | ¥ 2,480 | 5日内发货 |
产品描述 | Anti-MMBP Antibody (5B968) is a Rabbit antibody targeting MMBP. Anti-MMBP Antibody (5B968) can be used in WB. |
Ig Type | IgG |
交叉反应 | Escherichia coli |
验证活性 | 1. Western blot analysis of Maltose Binding Protein on recombinant MBP-tag protein lysates using anti-Maltose Binding Protein antibody at 1/1,000 dilution. |
应用 | |
推荐剂量 | WB: 1:1000-5000 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors frequently encode hybrid fusion proteins consisting in part of prokaryotic and in part, eukaryotic specified proteins. One such system utilizes maltose binding protein (MBP), the 370 amino acid product of the E. coli mal E gene. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. Expression systems utilizing the MBP fusion tag include pCG-806fx and pMal vectors. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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