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Anti-MID1IP1 Antibody (2E149)

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产品编号 TMAY-02677

Anti-MID1IP1 Antibody (2E149) 是一种 Rabbit 抗体,靶向 MID1IP1。Anti-MID1IP1 Antibody (2E149) 可用于 WB,ICC/IF。

Anti-MID1IP1 Antibody (2E149)

Anti-MID1IP1 Antibody (2E149)

Rating icon 还可以
产品编号 TMAY-02677

Anti-MID1IP1 Antibody (2E149) 是一种 Rabbit 抗体,靶向 MID1IP1。Anti-MID1IP1 Antibody (2E149) 可用于 WB,ICC/IF。

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产品介绍

生物活性
产品描述
Anti-MID1IP1 Antibody (2E149) is a Rabbit antibody targeting MID1IP1. Anti-MID1IP1 Antibody (2E149) can be used in WB,ICC/IF.
Ig Type
Monoclonal Rabbit IgG
克隆号
2E149
交叉反应
Human
验证活性
1. Anti-MID1IP1 rabbit monoclonal antibody at 1:500 dilution.

-Lane A: HepG2 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:20 kDa.

-Observed band size:20 kDa.

2. Immunofluorescence staining of MID1IP1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human MID1IP1 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoskeleton.
应用
WB,ICC/IF
推荐剂量
WB: 1:500-1:1000; ICC-IF: 1:20-1:100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human MID1IP1 (rh MID1IP1; TMPY-03495; Q9NPA3; Met1-His183).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景MID1IP1 (MID1 Interacting Protein 1) is a Protein Coding gene. The encoded protein belongs to the SPOT14 family. It is a homodimer in the absence of THRSP. MID1IP1 interacts with ACACA and ACACB. Its interaction with THRSP interferes with ACACA binding. It up-regulates ACACA enzyme activity and plays a role in the regulation of lipogenesis in the liver. MID1IP1 is required for efficient lipid biosynthesis, including triacylglycerol, diacylglycerol, and phospholipid. MID1IP1 is involved in the stabilization of microtubules. It is widely expressed in bone marrow, fat, and other tissues. Diseases associated with MID1IP1 include Gluten Allergy and Scoliosis. Among its related pathways are the Import of palmitoyl-CoA into the mitochondrial matrix and Metabolism.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Human MID1IP1 Protein (TMPY-03495)
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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