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Anti-MAP2K1/MEK2 Antibody (4G763) 是一种 Rabbit 抗体,靶向 MAP2K1/MEK2。Anti-MAP2K1/MEK2 Antibody (4G763) 可用于 WB,ICC/IF,IHC,IP。
Anti-MAP2K1/MEK2 Antibody (4G763) 是一种 Rabbit 抗体,靶向 MAP2K1/MEK2。Anti-MAP2K1/MEK2 Antibody (4G763) 可用于 WB,ICC/IF,IHC,IP。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,495 | 5日内发货 | |
100 μL | ¥ 2,490 | 5日内发货 |
产品描述 | Anti-MAP2K1/MEK2 Antibody (4G763) is a Rabbit antibody targeting MAP2K1/MEK2. Anti-MAP2K1/MEK2 Antibody (4G763) can be used in WB,ICC/IF,IHC,IP. |
别名 | PRKMK1, MKK1, MEK 1, MAPKK 1, MAPK/ERK kinase 1, MAP kinase kinase 1, ERK activator kinase 1, EC 2.7.12.2, Dual specificity mitogen-activated protein kinase kinase 1 |
Ig Type | IgG |
克隆号 | 4G763 |
交叉反应 | Human,Mouse,Rat,zebrafish |
验证活性 | 1. Western blot analysis of MEK1/2 on different cell lysates using anti-MEK1/2 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2, Lane 2: PC12, Lane 3: NIH/3T3. 2. Western blot analysis of MEK1/2 on hybrid fish (crucian-carp) brain tissue lysates using anti-MEK1/2 antibody at 1/500 dilution. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MEK1/2 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-MEK1/2 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-MEK1/2 antibody. Counter stained with hematoxylin. 6. ICC staining MEK1/2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining MEK1/2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
应用 | |
推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 44 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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