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Anti-LMAN2 Antibody (5W496)
还可以产品编号 TMAY-02354
Anti-LMAN2 Antibody (5W496) 是一种 Mouse 抗体，靶向 LMAN2。Anti-LMAN2 Antibody (5W496) 可用于 WB,ELISA,ELISA(Det),IP。
Anti-LMAN2 Antibody (5W496)
Anti-LMAN2 Antibody (5W496)
还可以产品编号 TMAY-02354
Anti-LMAN2 Antibody (5W496) 是一种 Mouse 抗体，靶向 LMAN2。Anti-LMAN2 Antibody (5W496) 可用于 WB,ELISA,ELISA(Det),IP。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-LMAN2 Antibody (5W496) is a Mouse antibody targeting LMAN2. Anti-LMAN2 Antibody (5W496) can be used in WB,ELISA,ELISA(Det),IP. |
| Ig Type | Monoclonal Mouse IgG2a |
| 克隆号 | 5W496 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-LMAN2 mouse monoclonal antibody at 1:500 dilution. -Lane A: Jurkat Whole Cell Lysate. -Lane B: Hela Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:40 kDa. -Observed band size:40 kDa(We are unsure as to the identity of these extra bands.) 2. LMAN2-His was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -2 µL anti-LMAN2-His mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-LMAN2-His mouse monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 40 kDa. -Observed band size: 40 kDa |
| 应用 | WB,ELISA,ELISA(Det),IP |
| 推荐剂量 | WB: 1:500-1:2000; ELISA: 1:1000-1:2000; IP: 4-6 μL/mg of lysate; ELISA(Det): 1:1000-1:10000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human LMAN2 / VIP36 (rh LMAN2 / VIP36; TMPY-02419; Q12907; Asp 45-Arg 322). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | LMAN2 (Lectin, Mannose Binding 2, also known as VIP36) is a Protein Coding gene. This gene encodes a type I transmembrane lectin that shuttles between the endoplasmic reticulum, the Golgi apparatus, and the plasma membrane. The encoded protein binds high mannose type glycoproteins and may facilitate their sorting, trafficking, and quality control. The L-type lectin LMAN2 appears to be specifically required for the accumulation of GPRC5B in the Golgi complex and restriction of GPRC5B transport along the exosomal pathway. This may occur due to interference with the adaptor protein GGA1-mediated trans-Golgi network-to-endosome transport of GPRC5B. A Golgi-traversing pathway for the exosomal release of the cargo protein GPRC5B in which CD2AP facilitates the entry and LMAN2 impedes the exit of the flux, respectively. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human LMAN2 / VIP36 protein (TMPY-02419) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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