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Anti-LGR-5 Antibody (8V603)
还可以产品编号 TMAH-00695
别名 LGR5, HG38, G-protein coupled receptor HG38, G-protein coupled receptor 67, G-protein coupled receptor 49, GPR67, GPR49
Anti-LGR-5 Antibody (8V603) 是一种抗体，靶向 LGR-5。Anti-LGR-5 Antibody (8V603) 可用于 ELISA, WB, FCM。
Anti-LGR-5 Antibody (8V603)
Anti-LGR-5 Antibody (8V603)
还可以产品编号 TMAH-00695 别名 LGR5, HG38, G-protein coupled receptor HG38, G-protein coupled receptor 67, G-protein coupled receptor 49, GPR67, GPR49
Anti-LGR-5 Antibody (8V603) 是一种抗体，靶向 LGR-5。Anti-LGR-5 Antibody (8V603) 可用于 ELISA, WB, FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,315 | 5日内发货 | |
| 100 μL | ¥ 2,185 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-LGR-5 Antibody (8V603) is an antibody targeting LGR-5. Anti-LGR-5 Antibody (8V603) can be used in ELISA, WB, FCM. |
| 别名 | LGR5, HG38, G-protein coupled receptor HG38, G-protein coupled receptor 67, G-protein coupled receptor 49, GPR67, GPR49 |
| Ig Type | Rabbit IgG |
| 克隆号 | 8V603 |
| 交叉反应 | Human, Rat |
| 验证活性 | 1. Western Blot -Positive WB detected in: SH-SY5Y whole cell lysate, Rat brain tissue -All lanes: LGR5 antibody at 1:1500 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 100, 98, 93 kDa -Observed band size: 100 kDa 2. Overlay histogram showing HepG2 cells stained with TMAH-00695 (red line) at 1:50. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| 应用 | ELISA, WB, FCM |
| 推荐剂量 | WB:1:500-1:5000; FCM:1:20-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Cell membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human LGR5/GPR49 |
| 抗原种属 | Human |
| 基因ID | 8549 |
| Uniprot ID | |
| 研究领域 | Cancer, Signal transduction, Stem cells |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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