Anti-Leukotriene A4 Hydrolase Antibody (2N373) is a Rabbit antibody targeting Leukotriene A4 Hydrolase. Anti-Leukotriene A4 Hydrolase Antibody (2N373) can be used in WB,ELISA,ICC/IF,IP.

Monoclonal Rabbit IgG

2N373

Human

1. Immunofluorescence staining of Human LTA4H in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human LTA4H monoclonal antibody (1:300) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

2. Anti-LTA4H rabbit monoclonal antibody at 1:500 dilution.

-Lane A: Hela Whole Cell Lysate.

-Lane B: A549 Whole Cell Lysate.

-Lane C: 293T Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:69 kDa.

-Observed band size:69 kDa.

3. LTA4H was immunoprecipitated using:

-Lane A:0.5 mg Hela Whole Cell Lysate.

-Lane B:0.5 mg 293T Whole Cell Lysate

-0.5 µL anti-LTA4H rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-LTA4H rabbit monoclonal antibody, at 1:500 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 110 kDa.

-Observed band size: 110 kDa

WB,ELISA,ICC/IF,IP

WB: 1:500-1:1000; ELISA: 1:25000-1:50000; ICC-IF: 1:100-1:500; IP: 1-2 μL/mg of lysate

Monoclonal

Unconjugated

Recombinant Human Leukotriene A4 Hydrolase / LTA4H protein (TMPY-00877)

Human