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Anti-Latexin Antibody (4S702)
还可以产品编号 TMAY-00473
Anti-Latexin Antibody (4S702) 是一种 Rabbit 抗体，靶向 Latexin。Anti-Latexin Antibody (4S702) 可用于 WB,ELISA,FCM,ICC/IF,IP。
Anti-Latexin Antibody (4S702)
Anti-Latexin Antibody (4S702)
还可以产品编号 TMAY-00473
Anti-Latexin Antibody (4S702) 是一种 Rabbit 抗体，靶向 Latexin。Anti-Latexin Antibody (4S702) 可用于 WB,ELISA,FCM,ICC/IF,IP。
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产品介绍
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偶联与修饰
抗原信息
| 产品描述 | Anti-Latexin Antibody (4S702) is a Rabbit antibody targeting Latexin. Anti-Latexin Antibody (4S702) can be used in WB,ELISA,FCM,ICC/IF,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 4S702 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunofluorescence staining of Human LXN in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human LXN monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. 2. Flow cytometric analysis of Human LXN expression on MCF-7 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human LXN, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. 3. Anti-LXN rabbit monoclonal antibody at 1:500 dilution. -Lane A: A549 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:26 kDa. -Observed band size:26 kDa. 4. LXN was immunoprecipitated using: -Lane A:0.5 mg A549 Whole Cell Lysate. -Lane B:0.5 mg Hela Whole Cell Lysate. -2 µL anti-LXN rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-LXN rabbit monoclonal antibody, at 1:200 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 25.7 kDa. -Observed band size: 25.7 kDa |
| 应用 | WB,ELISA,FCM,ICC/IF,IP |
| 推荐剂量 | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100; IP: 4-6 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human Latexin / LXN (rh Latexin / LXN; TMPY-00870; NP_064554.3; Glu 2-Glu 222) . |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Latexin, also known as endogenous carboxypeptidase inhibitor, tissue carboxypeptidase inhibitor, TCI, ECI, and LXN, is a cytoplasm protein that belongs to the protease inhibitor I47 (latexin) family. It is highly expressed in the heart, prostate, ovary, kidney, pancreas, and colon. Latexin / LXN is the only known endogenous specific inhibitor of zinc-dependent metallocarboxypeptidases (MCPs) present in mammalians so far. Latexin is originally identified as a molecular marker for the regional specification of the neocortex in development in rats. The 222 amino acid latexin in the human shows different expression distribution with high levels in heart, prostate, ovary, kidney, pancreas, and colon, but only moderate or low levels in other tissues including the brain. Latexin is also expressed at high levels and is inducible in macrophages in concert with other protease inhibitors and potential protease targets, and thus is suggested to play a role in inflammation and innate immunity pathways. Despite the non-detectable sequence similarity with plant and parasite inhibitors, Latexin is related to a human putative tumor suppressor protein, TIG1. Also, Latexin is implicated in Alzheimer's disease. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human Latexin / LXN Protein (TMPY-00870) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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