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Anti-LAMP2 Antibody (6M137)
还可以产品编号 TMAY-02602
Anti-LAMP2 Antibody (6M137) 是一种 Mouse 抗体，靶向 LAMP2。Anti-LAMP2 Antibody (6M137) 可用于 WB,ELISA,ICC/IF。
Anti-LAMP2 Antibody (6M137)
Anti-LAMP2 Antibody (6M137)
还可以产品编号 TMAY-02602
Anti-LAMP2 Antibody (6M137) 是一种 Mouse 抗体，靶向 LAMP2。Anti-LAMP2 Antibody (6M137) 可用于 WB,ELISA,ICC/IF。
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抗原信息
| 产品描述 | Anti-LAMP2 Antibody (6M137) is a Mouse antibody targeting LAMP2. Anti-LAMP2 Antibody (6M137) can be used in WB,ELISA,ICC/IF. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 6M137 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-LAMP2 mouse monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell Lysate. -Lane B: Jurkat Whole Cell Lysate. -Lane C: Hela Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:45 kDa. 2. Immunofluorescence staining of LAMP2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-human LAMP2 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to Cytoplasm. |
| 应用 | WB,ELISA,ICC/IF |
| 推荐剂量 | WB: 1:500-1:2000; ELISA: 1:1000-1:2000; ICC-IF: 1:20-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human LAMP2/CD107b (rh LAMP2/CD107b; TMPY-04115; NP_054701.1; Met1-Ile375). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | LAMP2 (Lysosomal Associated Membrane Protein 2) is a Protein Coding gene. LAMP2, also known as CD107b (Cluster of Differentiation 107b), is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. In humans, LAMP2, the causative gene of Danon disease, located on chromosome Xq24, encodes the lysosome-associated membrane protein-2 (LAMP-2). LAMP-2 deficiency, or Danon disease, is a rare X-linked lysosomal disease characterized by cardiomyopathy, vacuolar myopathy, and mental retardation. LAMP2 cardiomyopathy is an X-linked and highly progressive myocardial storage disorder associated with diminished survival, which clinically resembles sarcomeric hypertrophic cardiomyopathy. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human LAMP2/CD107b Protein (TMPY-04115) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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