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Anti-IL-33 Antibody (6H166)
还可以产品编号 TMAY-00705
Anti-IL-33 Antibody (6H166) 是一种 Mouse 抗体，靶向 IL-33。Anti-IL-33 Antibody (6H166) 可用于 WB,IP。
Anti-IL-33 Antibody (6H166)
Anti-IL-33 Antibody (6H166)
还可以产品编号 TMAY-00705
Anti-IL-33 Antibody (6H166) 是一种 Mouse 抗体，靶向 IL-33。Anti-IL-33 Antibody (6H166) 可用于 WB,IP。
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抗原信息
| 产品描述 | Anti-IL-33 Antibody (6H166) is a Mouse antibody targeting IL-33. Anti-IL-33 Antibody (6H166) can be used in WB,IP. |
| Ig Type | Monoclonal Mouse IgG2a |
| 克隆号 | 6H166 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-IL-33 mouse monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell Lysate. -Lane B: K562 Whole Cell Lysate. -Lane C: Jurkat Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:31 kDa. -Observed band size:25 kDa. 2. IL-33 was immunoprecipitated using: -Lane A:0.5 mg HeLa，K562，jurkat Whole Cell Lysate. -2 µL anti-IL-33 mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. -Primary antibody: -Anti-IL-33 mouse monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size: 31 kDa. -Observed band size:25 kDa |
| 应用 | WB,IP |
| 推荐剂量 | WB: 1:500-1:2000; IP: 0.5-2 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL-33 (rh IL-33; TMPY-00861; NP_254274.1; Ser112-Thr270). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Interleukin 33 (IL-33), also known as DVS27 or NF-HEV (Nuclear Factor from High Endothelial Venules), is a pro-inflammatory protein and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 protein is expressed highly and rather selectively by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyer's patches, and lymph nodes. IL-33 protein has transcriptional regulatory properties, and the researches suggested that IL-33 is a dual-function protein that might act both as a cytokine and as an intracellular nuclear factor. As a type 2 cytokines, IL-33 protein also play a pivotal role in helminthic infection and allergic disorders. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human IL-33 Protein (TMPY-00861) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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