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Anti-IkB alpha/NFKBIA Antibody (5T563)
还可以产品编号 TMAH-00815
别名 nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, NFKBI, MAD-3, IKBA, IkB α
Anti-IkB alpha/NFKBIA Antibody (5T563) 是一种抗体，靶向 IkB alpha/NFKBIA。Anti-IkB alpha/NFKBIA Antibody (5T563) 可用于 ELISA, WB, IHC, IP。
Anti-IkB alpha/NFKBIA Antibody (5T563)
Anti-IkB alpha/NFKBIA Antibody (5T563)
还可以产品编号 TMAH-00815 别名 nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, NFKBI, MAD-3, IKBA, IkB α
Anti-IkB alpha/NFKBIA Antibody (5T563) 是一种抗体，靶向 IkB alpha/NFKBIA。Anti-IkB alpha/NFKBIA Antibody (5T563) 可用于 ELISA, WB, IHC, IP。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,315 | 5日内发货 | |
| 100 μL | ¥ 2,180 | 5日内发货 |
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抗原信息
| 产品描述 | Anti-IkB alpha/NFKBIA Antibody (5T563) is an antibody targeting IkB alpha/NFKBIA. Anti-IkB alpha/NFKBIA Antibody (5T563) can be used in ELISA, WB, IHC, IP. |
| 别名 | nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, NFKBI, MAD-3, IKBA, IkB α |
| Ig Type | Rabbit IgG |
| 克隆号 | 5T563 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, PC3 whole cell lysate, THP-1 whole cell lysate -All lanes: NFKBIA antibody at 0.79μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 36 KDa -Observed band size: 36 KDa 2. IHC image of TMAH-00815 diluted at 1:79.75 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 3. Immunoprecipitating NFKBIA in HepG2 whole cell lysate -Lane 1: Rabbit control IgG instead of TMAH-00815 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) -Lane 2: TMAH-00815 (3μg) + HepG2 whole cell lysate (500μg) -Lane 3: HepG2 whole cell lysate (20μg) |
| 应用 | ELISA, WB, IHC, IP |
| 推荐剂量 | WB:1:500-1:5000; IHC:1:50-1:200; IP:1:200-1:1000. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Cytoplasm. Nucleus. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human NFKBIA |
| 抗原种属 | Human |
| 基因ID | 4792 |
| Uniprot ID | |
| 研究领域 | Signal Transduction |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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