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Anti-ICAM-1/CD54 Antibody-FITC (4H175)
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产品编号 TMAY-00663F
别名 P3.58, intercellular adhesion molecule 1, ICAM-1, CD54, BB2
Anti-ICAM-1/CD54 Antibody-FITC (4H175) 是一种 FITC-conjugated Mouse 抗体，靶向 ICAM-1/CD54。Anti-ICAM-1/CD54 Antibody-FITC (4H175) 可用于 FCM。
Anti-ICAM-1/CD54 Antibody-FITC (4H175)
331 Anti-ICAM-1/CD54 Antibody-FITC (4H175)
还可以产品编号 TMAY-00663F 别名 P3.58, intercellular adhesion molecule 1, ICAM-1, CD54, BB2
Anti-ICAM-1/CD54 Antibody-FITC (4H175) 是一种 FITC-conjugated Mouse 抗体，靶向 ICAM-1/CD54。Anti-ICAM-1/CD54 Antibody-FITC (4H175) 可用于 FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 25 T | ¥ 695 | 5日内发货 | |
| 100 T | ¥ 1,480 | 5日内发货 |
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产品介绍
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偶联与修饰
抗原信息
| 产品描述 | Anti-ICAM-1/CD54 Antibody-FITC (4H175) is a FITC-conjugated Mouse antibody targeting ICAM-1/CD54. Anti-ICAM-1/CD54 Antibody-FITC (4H175) can be used in FCM. |
| 别名 | P3.58, intercellular adhesion molecule 1, ICAM-1, CD54, BB2 |
| Ig Type | Mouse IgG1 |
| 克隆号 | 4H175 |
| 交叉反应 | Human |
| 验证活性 | 1. Profile of anti-ICAM1(CD54) reactivity on Daudi cells analyzed by flow cytometry. 2. Flow cytometric analysis of Human ICAM1 (CD54) in HUVEC cells. HUVEC cells (Human umbilical vein endothelial cells) were treated with TNF-α (50 ng/ml,18 hours). The cells were harvested and stained with FITC Mouse anti-Human ICAM1. |
| 应用 | FCM |
| 推荐剂量 | 10 μl/Test, 0.1 mg/ml |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ICAM-1 / CD54 (rh ICAM-1 / CD54; TMPY-01142; NP_000192.2; Met 1-Glu 480) and conjugated with FITC under optimum conditions, the unreacted FITC was removed. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | PBS solution containing 0.5% BSA and 0.09% sodium azide |
| 研究背景 | Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for some immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity. |
| 偶联 | FITC |
| 其他可选修饰符 | Unconjugated/APC/PerCP/PE |
| 可提供抗体种类 | 5 |
| 免疫原 | Recombinant Protein: Human ICAM-1 protein (TMPY-01142) |
| 抗原种属 | Human |
| 研究领域 | Neuroinflammation |
存储&运输
| 储存方式 | Store at 2°C-8°C for 12 months, do not freeze. Keep away from direct sunlight. Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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