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顶部生物活性储存和溶解信息计算器

Anti-Iba1 Antibody (4F442)

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Anti-Iba1 Antibody (4F442)
产品编号 TMAY-02699
别名 IRT-1, IRT1, IBA1, allograft inflammatory factor 1, AIF-1

Anti-Iba1 Antibody (4F442) 是一种 Rabbit 抗体，靶向 Iba1。Anti-Iba1 Antibody (4F442) 可用于 WB,ELISA,IP。

Anti-Iba1 Antibody (4F442)
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Anti-Iba1 Antibody (4F442)

Rating icon 还可以
Anti-Iba1 Antibody (4F442)
产品编号 TMAY-02699 别名 IRT-1, IRT1, IBA1, allograft inflammatory factor 1, AIF-1

Anti-Iba1 Antibody (4F442) 是一种 Rabbit 抗体，靶向 Iba1。Anti-Iba1 Antibody (4F442) 可用于 WB,ELISA,IP。

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100 μL
¥ 2,279
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产品介绍

生物活性
产品描述
Anti-Iba1 Antibody (4F442) is a Rabbit antibody targeting Iba1. Anti-Iba1 Antibody (4F442) can be used in WB,ELISA,IP.
别名IRT-1, IRT1, IBA1, allograft inflammatory factor 1, AIF-1
Ig Type
Rabbit IgG
克隆号
4F442
交叉反应
Human
验证活性
1. Anti-AIF1 rabbit monoclonal antibody at 1:500 dilution.
-Lane A: THP-1 Whole Cell Lysate.
-Lane B: HL-60 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:17 kDa.
-Observed band size:15 kDa.
2. AIF1 was immunoprecipitated using:
-Lane A:0.5 mg HepG2 Whole Cell Lysate.
-Lane B:0.5 mg THP-1 Whole Cell Lysate.
-2 µL anti-AIF1 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-AIF1 rabbit monoclonal antibody, at 1:100 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 17 kDa.
-Observed band size: 17 kDa
应用
WB,ELISA,IP
推荐剂量
WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human IBA1 / AIF1 (rh IBA1 / AIF1; TMPY-03337; P55008-1; Met1-Pro147).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景AIF1, also known as IBA1, is an actin-binding protein. AIF1 is expressed selectively in human macrophage-like cell lines, and in a subset of CD68(+) macrophages in the interstitial and perivascular spaces of human heart allografts. It is expressed in macrophages and neutrophils. AIF1 enhances membrane ruffling and RAC activation. AIF1 enhances the actin-bundling activity of LCP1. It also enhances lymphocyte migration. AIF1 may play a role in macrophage activation and function. It binds calcium and plays a role in RAC signaling and in phagocytosis. It promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: Human IBA1 / AIF1 Protein (TMPY-03337)
抗原种属
Human
研究领域
Calcium-binding Proteins and Related Molecules
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

计算器

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关键词

IRT 1IBA-1IBA 1AIF1AIF 1

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