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Anti-Iba1 Antibody (4F442)
还可以产品编号 TMAY-02699
Anti-Iba1 Antibody (4F442) 是一种 Rabbit 抗体，靶向 Iba1。Anti-Iba1 Antibody (4F442) 可用于 WB,ELISA,IP。
Anti-Iba1 Antibody (4F442)
Anti-Iba1 Antibody (4F442)
还可以产品编号 TMAY-02699
Anti-Iba1 Antibody (4F442) 是一种 Rabbit 抗体，靶向 Iba1。Anti-Iba1 Antibody (4F442) 可用于 WB,ELISA,IP。
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偶联与修饰
抗原信息
| 产品描述 | Anti-Iba1 Antibody (4F442) is a Rabbit antibody targeting Iba1. Anti-Iba1 Antibody (4F442) can be used in WB,ELISA,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 4F442 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-AIF1 rabbit monoclonal antibody at 1:500 dilution. -Lane A: THP-1 Whole Cell Lysate. -Lane B: HL-60 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:17 kDa. -Observed band size:15 kDa. 2. AIF1 was immunoprecipitated using: -Lane A:0.5 mg HepG2 Whole Cell Lysate. -Lane B:0.5 mg THP-1 Whole Cell Lysate. -2 µL anti-AIF1 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-AIF1 rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 17 kDa. -Observed band size: 17 kDa |
| 应用 | WB,ELISA,IP |
| 推荐剂量 | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human IBA1 / AIF1 (rh IBA1 / AIF1; TMPY-03337; P55008-1; Met1-Pro147). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | AIF1, also known as IBA1, is an actin-binding protein. AIF1 is expressed selectively in human macrophage-like cell lines, and in a subset of CD68(+) macrophages in the interstitial and perivascular spaces of human heart allografts. It is expressed in macrophages and neutrophils. AIF1 enhances membrane ruffling and RAC activation. AIF1 enhances the actin-bundling activity of LCP1. It also enhances lymphocyte migration. AIF1 may play a role in macrophage activation and function. It binds calcium and plays a role in RAC signaling and in phagocytosis. It promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human IBA1 / AIF1 Protein (TMPY-03337) |
| 抗原种属 | Human |
| 研究领域 | Calcium-binding Proteins and Related Molecules |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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