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Anti-Iba1 Antibody (3Y490)

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产品编号 TMAY-02698

Anti-Iba1 Antibody (3Y490) 是一种 Rabbit 抗体,靶向 Iba1。Anti-Iba1 Antibody (3Y490) 可用于 ELISA,IHC-P,ICC/IF。

Anti-Iba1 Antibody (3Y490)

Anti-Iba1 Antibody (3Y490)

Rating icon 还可以
产品编号 TMAY-02698

Anti-Iba1 Antibody (3Y490) 是一种 Rabbit 抗体,靶向 Iba1。Anti-Iba1 Antibody (3Y490) 可用于 ELISA,IHC-P,ICC/IF。

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产品介绍

生物活性
产品描述
Anti-Iba1 Antibody (3Y490) is a Rabbit antibody targeting Iba1. Anti-Iba1 Antibody (3Y490) can be used in ELISA,IHC-P,ICC/IF.
Ig Type
Monoclonal Rabbit IgG
克隆号
3Y490
交叉反应
Human
验证活性
1. Immunochemical staining of human AIF1 in human spleen with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).

2. Immunochemical staining of human AIF1 in human brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).

3. Immunofluorescence staining of AIF1 in K562 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human AIF1 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Cell membrane.
应用
ELISA,IHC-P,ICC/IF
推荐剂量
ELISA: 1:5000-1:10000; IHC-P: 1:100-1:500; ICC-IF: 1:20-1:100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human IBA1 / AIF1 (rh IBA1 / AIF1; TMPY-03337; P55008-1; Met1-Pro147).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景AIF1, also known as IBA1, is an actin-binding protein. AIF1 is expressed selectively in human macrophage-like cell lines, and in a subset of CD68(+) macrophages in the interstitial and perivascular spaces of human heart allografts. It is expressed in macrophages and neutrophils. AIF1 enhances membrane ruffling and RAC activation. AIF1 enhances the actin-bundling activity of LCP1. It also enhances lymphocyte migration. AIF1 may play a role in macrophage activation and function. It binds calcium and plays a role in RAC signaling and in phagocytosis. It promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Human IBA1 / AIF1 Protein (TMPY-03337)
抗原种属
Human
研究领域
Calcium-binding Proteins and Related Molecules
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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