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Anti-Iba1 Antibody (3Y490) 是一种 Rabbit 抗体,靶向 Iba1。Anti-Iba1 Antibody (3Y490) 可用于 ELISA,IHC-P,ICC/IF。
Anti-Iba1 Antibody (3Y490) 是一种 Rabbit 抗体,靶向 Iba1。Anti-Iba1 Antibody (3Y490) 可用于 ELISA,IHC-P,ICC/IF。
产品描述 | Anti-Iba1 Antibody (3Y490) is a Rabbit antibody targeting Iba1. Anti-Iba1 Antibody (3Y490) can be used in ELISA,IHC-P,ICC/IF. |
Ig Type | Monoclonal Rabbit IgG |
克隆号 | 3Y490 |
交叉反应 | Human |
验证活性 | 1. Immunochemical staining of human AIF1 in human spleen with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). 2. Immunochemical staining of human AIF1 in human brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). 3. Immunofluorescence staining of AIF1 in K562 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human AIF1 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Cell membrane. |
应用 | ELISA,IHC-P,ICC/IF |
推荐剂量 | ELISA: 1:5000-1:10000; IHC-P: 1:100-1:500; ICC-IF: 1:20-1:100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human IBA1 / AIF1 (rh IBA1 / AIF1; TMPY-03337; P55008-1; Met1-Pro147). |
纯化方式 | Protein A |
性状 | Liquid |
缓冲液 | 0.2 μm filtered solution in PBS |
研究背景 | AIF1, also known as IBA1, is an actin-binding protein. AIF1 is expressed selectively in human macrophage-like cell lines, and in a subset of CD68(+) macrophages in the interstitial and perivascular spaces of human heart allografts. It is expressed in macrophages and neutrophils. AIF1 enhances membrane ruffling and RAC activation. AIF1 enhances the actin-bundling activity of LCP1. It also enhances lymphocyte migration. AIF1 may play a role in macrophage activation and function. It binds calcium and plays a role in RAC signaling and in phagocytosis. It promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. |
偶联 | Unconjugated |
免疫原 | Recombinant Human IBA1 / AIF1 Protein (TMPY-03337) |
抗原种属 | Human |
研究领域 | Calcium-binding Proteins and Related Molecules |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
运输方式 | Shipping with blue ice. |
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