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Anti-HSP90AA1 Antibody (1U254)
还可以产品编号 TMAH-00572
Anti-HSP90AA1 Antibody (1U254) 是一种抗体，靶向 HSP90AA1。Anti-HSP90AA1 Antibody (1U254) 可用于 ELISA, WB, IHC, IF。
Anti-HSP90AA1 Antibody (1U254)
Anti-HSP90AA1 Antibody (1U254)
还可以产品编号 TMAH-00572
Anti-HSP90AA1 Antibody (1U254) 是一种抗体，靶向 HSP90AA1。Anti-HSP90AA1 Antibody (1U254) 可用于 ELISA, WB, IHC, IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,310 | 5日内发货 | |
| 100 μL | ¥ 2,195 | 5日内发货 |
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产品介绍
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抗原信息
| 产品描述 | Anti-HSP90AA1 Antibody (1U254) is an antibody targeting HSP90AA1. Anti-HSP90AA1 Antibody (1U254) can be used in ELISA, WB, IHC, IF. |
| Ig Type | Rabbit IgG |
| 克隆号 | 1U254 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in: Jurkat whole cell lysate -All lanes: Hsp90 alpha antibody at 1.9μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 85, 99 KDa -Observed band size: 90 KDa 2. IHC image of TMAH-00572 diluted at 1:190 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 3. Immunofluorescence staining of NIH/3T3 cells with TMAH-00572 at 1:63, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| 应用 | ELISA, WB, IHC, IF |
| 推荐剂量 | WB:1:500-1:5000; IHC:1:50-1:200; IF:1:20-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Nucleus. Cytoplasm. Melanosome. Cell membrane. Mitochondrion. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle. Plays a critical role in mitochondrial import, delivers preproteins to the mitochondrial import receptor TOMM70. Apart from its chaperone activity, it also plays a role in the regulation of the transcription machinery. HSP90 and its co-chaperones modulate transcription at least at three different levels. In the first place, they alter the steady-state levels of certain transcription factors in response to various physiological cues(PubMed:25973397). Second, they modulate the activity of certain epigenetic modifiers, such as histone deacetylases or DNA methyl transferases, and thereby respond to the change in the environment. Third, they participate in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression. Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antagonizes STUB1-mediated inhibition of TGF-beta signaling via inhibition of STUB1-mediated SMAD3 ubiquitination and degradation. Mediates the association of TOMM70 with IRF3 or TBK1 in mitochodria outer membrane which promotes host antiviral response. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human HSP90AA1 |
| 抗原种属 | Human |
| 基因ID | 3320 |
| Uniprot ID | |
| 研究领域 | Signal Transduction |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
关键词
Renal carcinoma antigen NY-REN-38Lipopolysaccharide-associated protein 2 (LAP-2;LPS-associated protein 2)LAP-2LAP2HSPCAHSPC1HSPC 1HSP90AA1HSP90AHSP90 AA1HSP86HSP 86Heat shock protein HSP 90-αHeat shock protein HSP 90-alphaHeat shock protein HSP 90-aHeat shock protein family C member 1Heat shock 86 kDa (HSP 86;HSP86)
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