Anti-HOXc8 Polyclonal Antibody is a Rabbit antibody targeting HOXc8. Anti-HOXc8 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Rat (predicted:Mouse,Pig,Cow,Horse)

1. Sample: liver (Rat) Lysate at 40 μg
Primary: Anti-HOXc8 (TMAB-07254) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 27 kD
2. Sample:
Brain (Rat) lysate at 30 μg;
liver (Rat) lysate at 30 μg;
Primary: Anti-HOXc8 (TMAB-07254) at 1: 200;
Secondary: HRP conjugated Goat-Anti-Rabbit IgGat 1: 3000;
Predicted band size: 27 kD
Observed band size: 27 kD, 43 kD
3. Paraformaldehyde-fixed, paraffin embedded Rat small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer normal goat serum at 37°C for 30 min; Antibody incubation with (Hox-3.1) Polyclonal Antibody, Unconjugated (TMAB-07254) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Hox-3.1) Polyclonal Antibody, Unconjugated (TMAB-07254) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Histone H3) Polyclonal Antibody, Unconjugated (TMAB-07254) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human HOXc8

Human

HOXC8

Homeobox protein Hox-C8

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.