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Anti-HNMT Antibody (7C164)
还可以产品编号 TMAY-02476
Anti-HNMT Antibody (7C164) 是一种 Rabbit 抗体，靶向 HNMT。Anti-HNMT Antibody (7C164) 可用于 WB,ELISA,IP。
Anti-HNMT Antibody (7C164)
Anti-HNMT Antibody (7C164)
还可以产品编号 TMAY-02476
Anti-HNMT Antibody (7C164) 是一种 Rabbit 抗体，靶向 HNMT。Anti-HNMT Antibody (7C164) 可用于 WB,ELISA,IP。
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产品介绍
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偶联与修饰
抗原信息
| 产品描述 | Anti-HNMT Antibody (7C164) is a Rabbit antibody targeting HNMT. Anti-HNMT Antibody (7C164) can be used in WB,ELISA,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 7C164 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-HNMT rabbit monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:33 kDa. -Observed band size:33 kDa. 2. HNMT was immunoprecipitated using: -Lane A:0.5 mg HepG2 Whole Cell Lysate. -Lane B:0.5 mg Hela Whole Cell Lysate. -2 µL anti-HNMT rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-HNMT rabbit monoclonal antibody, at 1:200 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 33 kDa. -Observed band size: 33 kDa |
| 应用 | WB,ELISA,IP |
| 推荐剂量 | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 0.5-2 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human HNMT (rh HNMT; TMPY-02340; AAH20677.1; Met1-Ala292). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | HNMT (Histamine N-methyltransferase) is a Protein Coding gene. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. HNMT, the major enzyme for the metabolism of histamine in the rat brain, is potently inhibited by 9-amino-1,2,3,4-tetrahydroacridine (tacrine). Methylation is an important pathway in the biotransformation of many drugs, neurotransmitters, and xenobiotic compounds. Histamine N-methyltransferase (HNMT) catalyzes the N tau-methylation of histamine and structurally related compounds. Histamine N-methyltransferase (HNMT) is believed to be the sole pathway for termination of the neurotransmitter action of histamine in the mammalian brain. That highlights the importance of the inclusion of HNMT for genetic testing of individuals presenting with intellectual disability. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human HNMT protein (TMPY-02340) |
| 抗原种属 | Human |
| 研究领域 | Neurotransmitter Associated Enzymes |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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