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Anti-HNMT Antibody (7C164)

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Anti-HNMT Antibody (7C164)
产品编号 TMAY-02476
别名 HNMT-S2, HNMT-S1, HMT, histamine N-methyltransferase

Anti-HNMT Antibody (7C164) 是一种 Rabbit 抗体，靶向 HNMT。Anti-HNMT Antibody (7C164) 可用于 WB,ELISA,IP。

Anti-HNMT Antibody (7C164)
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Anti-HNMT Antibody (7C164)

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Anti-HNMT Antibody (7C164)
产品编号 TMAY-02476 别名 HNMT-S2, HNMT-S1, HMT, histamine N-methyltransferase

Anti-HNMT Antibody (7C164) 是一种 Rabbit 抗体，靶向 HNMT。Anti-HNMT Antibody (7C164) 可用于 WB,ELISA,IP。

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100 μL
¥ 2,279
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产品介绍

生物活性
产品描述
Anti-HNMT Antibody (7C164) is a Rabbit antibody targeting HNMT. Anti-HNMT Antibody (7C164) can be used in WB,ELISA,IP.
别名HNMT-S2, HNMT-S1, HMT, histamine N-methyltransferase
Ig Type
Rabbit IgG
克隆号
7C164
交叉反应
Human
验证活性
1. Anti-HNMT rabbit monoclonal antibody at 1:500 dilution.
-Lane A: HepG2 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:33 kDa.
-Observed band size:33 kDa.
2. HNMT was immunoprecipitated using:
-Lane A:0.5 mg HepG2 Whole Cell Lysate.
-Lane B:0.5 mg Hela Whole Cell Lysate.
-2 µL anti-HNMT rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-HNMT rabbit monoclonal antibody, at 1:200 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 33 kDa.
-Observed band size: 33 kDa
应用
WB,ELISA,IP
推荐剂量
WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 0.5-2 μL/mg of lysate
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human HNMT (rh HNMT; TMPY-02340; AAH20677.1; Met1-Ala292).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景HNMT (Histamine N-methyltransferase) is a Protein Coding gene. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. HNMT, the major enzyme for the metabolism of histamine in the rat brain, is potently inhibited by 9-amino-1,2,3,4-tetrahydroacridine (tacrine). Methylation is an important pathway in the biotransformation of many drugs, neurotransmitters, and xenobiotic compounds. Histamine N-methyltransferase (HNMT) catalyzes the N tau-methylation of histamine and structurally related compounds. Histamine N-methyltransferase (HNMT) is believed to be the sole pathway for termination of the neurotransmitter action of histamine in the mammalian brain. That highlights the importance of the inclusion of HNMT for genetic testing of individuals presenting with intellectual disability.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: Human HNMT protein (TMPY-02340)
抗原种属
Human
研究领域
Neurotransmitter Associated Enzymes
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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