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Anti-HMGCS2 Antibody (2I93)

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Anti-HMGCS2 Antibody (2I93)
产品编号 TMAH-00561
别名 mitochondrial, Hydroxymethylglutaryl-CoA synthase, HMGCS 2, HMG-CoA synthase, EC 2.3.3.10, 3-hydroxy-3-methylglutaryl coenzyme A synthase

Anti-HMGCS2 Antibody (2I93) 是一种抗体,靶向 HMGCS2。Anti-HMGCS2 Antibody (2I93) 可用于 ELISA, WB, IHC。

Anti-HMGCS2 Antibody (2I93)

Anti-HMGCS2 Antibody (2I93)

Rating icon 还可以
Anti-HMGCS2 Antibody (2I93)
产品编号 TMAH-00561 别名 mitochondrial, Hydroxymethylglutaryl-CoA synthase, HMGCS 2, HMG-CoA synthase, EC 2.3.3.10, 3-hydroxy-3-methylglutaryl coenzyme A synthase

Anti-HMGCS2 Antibody (2I93) 是一种抗体,靶向 HMGCS2。Anti-HMGCS2 Antibody (2I93) 可用于 ELISA, WB, IHC。

规格价格库存数量
50 μL
¥ 1,315
5日内发货
100 μL
¥ 2,185
5日内发货
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产品介绍

生物活性
产品描述
Anti-HMGCS2 Antibody (2I93) is an antibody targeting HMGCS2. Anti-HMGCS2 Antibody (2I93) can be used in ELISA, WB, IHC.
别名mitochondrial, Hydroxymethylglutaryl-CoA synthase, HMGCS 2, HMG-CoA synthase, EC 2.3.3.10, 3-hydroxy-3-methylglutaryl coenzyme A synthase
Ig Type
Rabbit IgG
克隆号
2I93
交叉反应
Human
验证活性
1. Western Blot
-Positive WB detected in: A549 whole cell lysate, Ntera-2 whole cell lysate
-All lanes: HMGCS2 antibody at 1:500
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 57, 53, 51 kDa
-Observed band size: 55 kDa
2. IHC image of TMAH-00561 diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
3. IHC image of TMAH-00561 diluted at 1:100 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
应用
推荐剂量
WB:1:500-1:2000; IHC:1:50-1:200.
抗体种类
Monoclonal
亚细胞定位Mitochondrion.
组织特异性Expression in liver is 200-fold higher than in any other tissue. Low expression in colon, kidney, testis, and pancreas. Very low expression in heart and skeletal muscle. Not detected in brain.; [Isoform 3]: Highest expression detected in heart and skeleta
构建方式Recombinant Antibody
纯化方式Affinity-chromatography
性状Liquid
缓冲液Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景Catalyzes the first irreversible step in ketogenesis, condensing acetyl-CoA to acetoacetyl-CoA to form HMG-CoA, which is converted by HMG-CoA reductase (HMGCR) into mevalonate.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
A synthetic peptide: Human HMGCS2
抗原种属
Human
基因名称
HMGCS2
基因ID
Uniprot ID
研究领域
Cancer, Cardiovascular, Metabolism, Signal transduction
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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