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Anti-HMGCS2 Antibody (2I93) 是一种抗体,靶向 HMGCS2。Anti-HMGCS2 Antibody (2I93) 可用于 ELISA, WB, IHC。
Anti-HMGCS2 Antibody (2I93) 是一种抗体,靶向 HMGCS2。Anti-HMGCS2 Antibody (2I93) 可用于 ELISA, WB, IHC。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,315 | 5日内发货 | |
100 μL | ¥ 2,185 | 5日内发货 |
产品描述 | Anti-HMGCS2 Antibody (2I93) is an antibody targeting HMGCS2. Anti-HMGCS2 Antibody (2I93) can be used in ELISA, WB, IHC. |
别名 | mitochondrial, Hydroxymethylglutaryl-CoA synthase, HMGCS 2, HMG-CoA synthase, EC 2.3.3.10, 3-hydroxy-3-methylglutaryl coenzyme A synthase |
Ig Type | Rabbit IgG |
克隆号 | 2I93 |
交叉反应 | Human |
验证活性 | 1. Western Blot -Positive WB detected in: A549 whole cell lysate, Ntera-2 whole cell lysate -All lanes: HMGCS2 antibody at 1:500 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 57, 53, 51 kDa -Observed band size: 55 kDa 2. IHC image of TMAH-00561 diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. 3. IHC image of TMAH-00561 diluted at 1:100 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. |
应用 | |
推荐剂量 | WB:1:500-1:2000; IHC:1:50-1:200. |
抗体种类 | Monoclonal |
亚细胞定位 | Mitochondrion. |
组织特异性 | Expression in liver is 200-fold higher than in any other tissue. Low expression in colon, kidney, testis, and pancreas. Very low expression in heart and skeletal muscle. Not detected in brain.; [Isoform 3]: Highest expression detected in heart and skeleta |
构建方式 | Recombinant Antibody |
纯化方式 | Affinity-chromatography |
性状 | Liquid |
缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | Catalyzes the first irreversible step in ketogenesis, condensing acetyl-CoA to acetoacetyl-CoA to form HMG-CoA, which is converted by HMG-CoA reductase (HMGCR) into mevalonate. |
偶联 | Unconjugated |
免疫原 | A synthetic peptide: Human HMGCS2 |
抗原种属 | Human |
基因名称 | HMGCS2 |
基因ID | |
Uniprot ID | |
研究领域 | Cancer, Cardiovascular, Metabolism, Signal transduction |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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