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Anti-Histone H3.3 Antibody (5A720)

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Anti-Histone H3.3 Antibody (5A720)
产品编号 TMAC-01951
别名 MGC87783, MGC87782, Histone H3.B, Histone H3.A, Histone H3.3Q, H3f3b, H3F3A, H3F3, H33_HUMAN, H3.3B, H3.3A, H3.3, H3 histone family 3B, H3 histone family 3A

Anti-Histone H3.3 Antibody (5A720) 是一种 Rabbit 抗体,靶向 Histone H3.3。Anti-Histone H3.3 Antibody (5A720) 可用于 WB,ICC/IF,IHC,ChIP。

Anti-Histone H3.3 Antibody (5A720)

Anti-Histone H3.3 Antibody (5A720)

Rating icon 还可以
Anti-Histone H3.3 Antibody (5A720)
产品编号 TMAC-01951 别名 MGC87783, MGC87782, Histone H3.B, Histone H3.A, Histone H3.3Q, H3f3b, H3F3A, H3F3, H33_HUMAN, H3.3B, H3.3A, H3.3, H3 histone family 3B, H3 histone family 3A

Anti-Histone H3.3 Antibody (5A720) 是一种 Rabbit 抗体,靶向 Histone H3.3。Anti-Histone H3.3 Antibody (5A720) 可用于 WB,ICC/IF,IHC,ChIP。

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50 μL
¥ 1,485
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100 μL
¥ 2,495
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产品介绍

生物活性
产品描述
Anti-Histone H3.3 Antibody (5A720) is a Rabbit antibody targeting Histone H3.3. Anti-Histone H3.3 Antibody (5A720) can be used in WB,ICC/IF,IHC,ChIP.
别名MGC87783, MGC87782, Histone H3.B, Histone H3.A, Histone H3.3Q, H3f3b, H3F3A, H3F3, H33_HUMAN, H3.3B, H3.3A, H3.3, H3 histone family 3B, H3 histone family 3A
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of Histone H3.3 on BT-20 cell lysates using anti-Histone H3.3 antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin.
6. ICC staining Histone H3.3 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining Histone H3.3 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
应用
推荐剂量
WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
化学信息
分子量Theoretical: 15 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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