Anti-His tag Antibody (3T670) is a Mouse antibody targeting His tag. Anti-His tag Antibody (3T670) can be used in ELISA, FCM, ICC/IF, IP, WB, TR-FRET.

IgG1,κ

3T670

His tags localized at the C-terminal, N-terminal, and internal region of fusion proteins

1. Comparative His-tag detection by Western blot was performed using Anti-His tag Antibody (3T670) (TMAS-00127) and Mouse Anti-His mAb (Competitor B). Both antibodies were used to probe the same cell lysates containing His-tagged fusion protein. The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody.
2. Immunoprecipitates from cell lysates containing His-tagged fusion protein were analyzed by Western blot using Anti-His tag Antibody (3T670) (TMAS-00127).
Lane 1. Positive control containing His-tagged fusion protein.
Lane 2. Negative control – IP with isotype control antibody.
Lane 3. Immunoprecipitation with Anti-His tag Antibody (3T670) (TMAS-00127).
3. His-tag was detected in Multiple Tag Cell Lysate by Western blot using Anti-His tag Antibody (3T670) (TMAS-00127). The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody.
His-tagged fusion protein:
Predicted MW: 52 kDa
Observed MW: 52 kDa
4. Comparative His-tag detection by Dot blot was performed using Anti-His tag Antibody (3T670) (TMAS-00127) and two Mouse Anti-His mAbs (Competitor 1, Competitor 2). All three antibodies were used to probe the same samples containing His-tagged fusion protein. The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody.
5. Lot-to-lot consistency of antibody performance was analyzed for 4 batches of Anti-His tag Antibody (3T670) (TMAS-00127) by Western blot. The results show that the antibody-generated signal remains consistent from Lot-to-Lot.Antibodies from all four lots were used to probe samples of the same His-tagged fusion protein.The signal was developed with IRDyeTM800 Conjugated Goat Anti-Mouse IgG.
6. Detection of His-tag in CHO cells transfected with His-tagged protein (Green), and non-transfected CHO cells (Black) by flow cytometry using Anti-His tag Antibody (3T670) (TMAS-00127). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.
7. Comparative His-tag detection by Western blot was performed using Anti-His tag Antibody (3T670) (TMAS-00127) and Mouse Anti-His mAb (Competitor A). Both antibodies were used to probe the same sample containing overexpressed His-tagged fusion protein.
8. Detection of N- or C-terminal His tags in recombinant fusion proteins were analyzed by Western blot using Anti-His tag Antibody (3T670) (TMAS-00127).
Lane 1: N-terminal His-tagged fusion protein.
Lane 2: C-terminal His-tagged fusion protein.
The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody.
The results show that Anti-His tag Antibody (3T670) (TMAS-00127) can recognize N-terminal and C-terminal His-tagged proteins.
9. BLI (Biolayer interferometry) binding affinity measurements of Anti-His tag Antibody (3T670) (TMAS-00127) to different His-tagged fusion proteins. The results show that Anti-His tag Antibody (3T670) (TMAS-00127) is able to recognise the His tag at different positions in the protein.

ELISA: 0.05-0.2 µg/ml; FCM: 1 µg/ml; ICC/IF: 1 µg/ml; IP: 1 µg/ml; WB: 0.1-0.2 µg/ml; TR-FRET: User-optimized

Monoclonal

Unconjugated

HRP/Biotin/FITC/iFluor 488/iFluor 555/iFluor 647/PE

8

KLH conjugated synthetic peptide: HHHHHH