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Anti-GFP Antibody (2A799)

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货号 TMAB-00764

别名 GFP Protein

Anti-GFP Antibody (2A799) 是一种 Mouse 抗体，靶向 GFP。Anti-GFP Antibody (2A799) 可用于 ELISA, FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB。

Anti-GFP Antibody (2A799)

Anti-GFP Antibody (2A799)

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Rating icon 还可以
货号 TMAB-00764 别名 GFP Protein

Anti-GFP Antibody (2A799) 是一种 Mouse 抗体，靶向 GFP。Anti-GFP Antibody (2A799) 可用于 ELISA, FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB。

规格价格库存数量
100 μL
¥ 560
5日内发货
200 μL
¥ 970
5日内发货
500 μL
¥ 2,270
5日内发货
1 mL
¥ 4,260
5日内发货
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产品介绍

生物活性
产品描述
Anti-GFP Antibody (2A799) is a Mouse antibody targeting GFP. Anti-GFP Antibody (2A799) can be used in ELISA, FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB.
别名GFP Protein
Ig Type
IgG
克隆号
2A799
反应种属
Species independent
验证活性
1. Transformed (GFP or EV) E. coli cells lysates were subjected to SDS-PAGE followed by WB with TMAB-00764 (Anti-GFP) at dilution of 1:1,000,000 incubated at 4°C overnight.
2. Sample:
GFP-tagged fusion protein Overexpression E.coli Lysate at 4 μg
Primary:
Anti-GFP-Tag (TMAB-00764) at 1/1000000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 30 kDa
3. Sample:
GFP-Tagged Fusion Protein Overexpression E.coli Lysate at 2 μg
Primary: Anti-GST tag (TMAB-00764) at 1/5000 ~ 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
verifiedActivityverifiedActivityverifiedActivity
应用ELISAFCMICC/IFIFIHC-FrIHC-PWB
推荐剂量
ELISA=1:1000-5000; FCM=1 μg/Test; ICC/IF=1:100-500; IF=1:200-1000; IHC-Fr=1:200-1000; IHC-P=1:200-1000; WB=1:50000-500000
抗体种类
Monoclonal
宿主来源Mouse
组织特异性Photocytes.
构建方式Hybridoma Monoclonal Antibody
纯化方式Protein G purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1 mg/mL
研究背景Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.
抗原信息
免疫原
Recombinant Protein: Green Fluorescence Protein
基因名称
GFP
蛋白名称
Green fluorescent protein
研究领域
GFP
功能
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin
储存&溶解度
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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