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Anti-GAPDH Antibody (1V316)
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产品编号 TMAC-01615
别名 HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, GAPD, G3PD
Anti-GAPDH Antibody (1V316) 是一种 Mouse 抗体，靶向 GAPDH。Anti-GAPDH Antibody (1V316) 可用于 WB,ICC。
Anti-GAPDH Antibody (1V316)
Anti-GAPDH Antibody (1V316)
还可以产品编号 TMAC-01615 别名 HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, GAPD, G3PD
Anti-GAPDH Antibody (1V316) 是一种 Mouse 抗体，靶向 GAPDH。Anti-GAPDH Antibody (1V316) 可用于 WB,ICC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,995 | 5日内发货 | |
| 100 μL | ¥ 2,995 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-GAPDH Antibody (1V316) is a Mouse antibody targeting GAPDH. Anti-GAPDH Antibody (1V316) can be used in WB,ICC. |
| 别名 | HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, GAPD, G3PD |
| 交叉反应 | Human,Mouse,Rat,zebrafish,rabbit |
| 验证活性 | 1. Western blot analysis of GAPDH on different cell lysates using anti-GAPDH antibody at 1/5000 dilution. Positive control: Lane 1: HepG2, Lane 2: Hela, Lane 3: PC12, Lane 4: NIH/3T3, Lane 5: MCF-7, Lane 6: Rabbit liver, Lane 7: Zebrafish. 2. ICC staining GAPDH in F9 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining GAPDH in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining GAPDH in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| 应用 | |
| 推荐剂量 | WB: 1:5000-10000; ICC: 1:200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | ProL affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. It participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. GAPDH is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. |
| 偶联 | Unconjugated |
| 免疫原 | Peptide |
| Uniprot ID |
| 分子量 | Theoretical: 36 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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