Anti-FTH Antibody (9C880)

Name: Anti-FTH Antibody (9C880)
Brand: TargetMol
SKU: TMAB-00718
Rating: 4.5 (1 reviews)

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产品编号 TMAB-00718

Anti-FTH Antibody (9C880) 是一种 Rabbit 抗体，靶向 FTH。Anti-FTH Antibody (9C880) 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-FTH Antibody (9C880)

还可以

产品编号 TMAB-00718

Anti-FTH Antibody (9C880) 是一种 Rabbit 抗体，靶向 FTH。Anti-FTH Antibody (9C880) 可用于 WB,IHC-P,IHC-Fr,IF。

规格	价格	库存
25 μL	¥ 795	5日内发货
50 μL	¥ 1,395	5日内发货
100 μL	¥ 2,490	5日内发货

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产品描述	Anti-FTH Antibody (9C880) is a Rabbit antibody targeting FTH. Anti-FTH Antibody (9C880) can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type	IgG
克隆号	9C880
交叉反应	Human
验证活性	1. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Ferritin Heavy Chain Monoclonal Antibody, Unconjugated (TMAB-00718) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Ferritin Heavy Chain Monoclonal Antibody, Unconjugated (TMAB-00718) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Ferritin Heavy Chain Monoclonal Antibody, Unconjugated (TMAB-00718) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Ferritin Heavy Chain Monoclonal Antibody, Unconjugated (TMAB-00718) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Ferritin Heavy Chain Monoclonal Antibody, Unconjugated (TMAB-00718) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Ferritin Heavy Chain Monoclonal Antibody, Unconjugated (TMAB-00718) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Ferritin Heavy Chain Monoclonal Antibody, Unconjugated (TMAB-00718) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Ferritin Heavy Chain Monoclonal Antibody, Unconjugated (TMAB-00718) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Ferritin Heavy Chain Monoclonal Antibody, Unconjugated (TMAB-00718) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 10. 25 μg total protein per Lane of various lysates probed with Ferritin Heavy Chain monoclonal antibody, unconjugated (TMAB-00718) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
应用	WB IHC-P IHC-Fr IF
推荐剂量	WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类	Monoclonal
宿主来源	Rabbit
亚细胞定位	Cytoplasmic.
构建方式	Recombinant Antibody
纯化方式	Protein A purified
性状	Liquid
缓冲液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景	Mammalian ferritins consist of 24 subunits made up of two types of poly-peptide chains, ferritin heavy chain and ferritin light chain, which each have unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of FeII, whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of FeIII. The most prominent role of mamma-lian ferritins is to provide iron-buffering capacity to cells. In addition to iron buffering, heavy chain ferritin is also involved in the regulation of thymidine biosynthesis via increased expression of cytoplasmic serine hydroxymethyltransferase, which is a limiting factor in thymidylate synthesis in MCF-7 cells. Light chain ferritin is involved in cataracts by at least two mechanisms: hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed; and oxidative stress, an important factor in the development of aging-related cataracts.

抗原信息

免疫原	A synthesized peptide: human Ferritin heavy chain
抗原种属	Human
基因ID	2495
Uniprot ID	P02794
研究领域	Serum Proteins,Vitamins / minerals,Neurodegenerative disease,Alzheimer's disease,Vitamins / Minerals