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Anti-FTH Antibody (9C880) 是一种 Rabbit 抗体,靶向 FTH。Anti-FTH Antibody (9C880) 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-FTH Antibody (9C880) 是一种 Rabbit 抗体,靶向 FTH。Anti-FTH Antibody (9C880) 可用于 WB,IHC-P,IHC-Fr,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
25 μL | ¥ 795 | 5日内发货 | |
50 μL | ¥ 1,395 | 5日内发货 | |
100 μL | ¥ 2,490 | 5日内发货 |
产品描述 | Anti-FTH Antibody (9C880) is a Rabbit antibody targeting FTH. Anti-FTH Antibody (9C880) can be used in WB,IHC-P,IHC-Fr,IF. |
别名 | FTHL6, FTH1, FTH, Ferritin heavy chain, Ferritin H subunit, Cell proliferation-inducing gene 15 protein |
Ig Type | IgG |
克隆号 | 9C880 |
交叉反应 | Human |
验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Ferritin Heavy Chain Monoclonal Antibody, Unconjugated (TMAB-00718) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Ferritin Heavy Chain Monoclonal Antibody, Unconjugated (TMAB-00718) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Ferritin Heavy Chain Monoclonal Antibody, Unconjugated (TMAB-00718) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Ferritin Heavy Chain Monoclonal Antibody, Unconjugated (TMAB-00718) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Ferritin Heavy Chain Monoclonal Antibody, Unconjugated (TMAB-00718) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Ferritin Heavy Chain Monoclonal Antibody, Unconjugated (TMAB-00718) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Ferritin Heavy Chain Monoclonal Antibody, Unconjugated (TMAB-00718) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Ferritin Heavy Chain Monoclonal Antibody, Unconjugated (TMAB-00718) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Ferritin Heavy Chain Monoclonal Antibody, Unconjugated (TMAB-00718) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 10. 25 μg total protein per Lane of various lysates probed with Ferritin Heavy Chain monoclonal antibody, unconjugated (TMAB-00718) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
亚细胞定位 | Cytoplasmic. |
组织特异性 | In human liver the heavy chain is the major chain. |
构建方式 | Recombinant Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
浓度 | 1 mg/mL |
研究背景 | Mammalian ferritins consist of 24 subunits made up of two types of poly-peptide chains, ferritin heavy chain and ferritin light chain, which each have unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of FeII, whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of FeIII. The most prominent role of mamma-lian ferritins is to provide iron-buffering capacity to cells. In addition to iron buffering, heavy chain ferritin is also involved in the regulation of thymidine biosynthesis via increased expression of cytoplasmic serine hydroxymethyltransferase, which is a limiting factor in thymidylate synthesis in MCF-7 cells. Light chain ferritin is involved in cataracts by at least two mechanisms: hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed; and oxidative stress, an important factor in the development of aging-related cataracts. |
免疫原 | A synthesized peptide: human Ferritin heavy chain |
抗原种属 | Human |
基因名称 | FTH1 |
基因ID | |
蛋白名称 | Ferritin heavy chain |
Uniprot ID | |
研究领域 | Serum Proteins,Vitamins / minerals,Neurodegenerative disease,Alzheimer's disease,Vitamins / Minerals |
功能 | Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. |
分子量 | Theoretical: 20 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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