Anti-FASN Antibody (1K266) is a Mouse antibody targeting FASN. Anti-FASN Antibody (1K266) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG1κ

1K266

Human (predicted:Mouse)

1. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (FASN) Monoclonal Antibody, Unconjugated (TMAB-00658) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (FASN) Monoclonal Antibody, Unconjugated (TMAB-00658) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (FASN) Monoclonal Antibody, Unconjugated (TMAB-00658) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (FASN) Monoclonal Antibody, Unconjugated (TMAB-00658) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
5. 25 μg total protein per Lane of various lysates probed with FASN monoclonal antibody, unconjugated (TMAB-00658) at 1:4000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.

WB: 1:500-5000; IHC-P: 1:100-500; IHC-Fr: 1:20-200; ICC/IF: 1:100-500; IF: 1:20-200

Monoclonal

KLH conjugated synthetic peptide: human FASN

Human

Integration of energy metabolism,Metabolism of lipids and lipoproteins,Enzymes,Synthesis,Energy Metabolism,Integration of energy,Lipid metabolism,Fatty acid oxidation,Cancer,Obesity,Metabolism,Energy Metabolism,Lipid metabolism