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Anti-ETHE1 Antibody (6K797)

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Anti-ETHE1 Antibody (6K797)
产品编号 TMAY-02767
别名 YF13H12, HSCO, ethylmalonic encephalopathy 1

Anti-ETHE1 Antibody (6K797) 是一种 Rabbit 抗体，靶向 ETHE1。Anti-ETHE1 Antibody (6K797) 可用于 WB,ELISA,IP。

Anti-ETHE1 Antibody (6K797)

Anti-ETHE1 Antibody (6K797)

Rating icon 还可以
Anti-ETHE1 Antibody (6K797)
产品编号 TMAY-02767 别名 YF13H12, HSCO, ethylmalonic encephalopathy 1

Anti-ETHE1 Antibody (6K797) 是一种 Rabbit 抗体，靶向 ETHE1。Anti-ETHE1 Antibody (6K797) 可用于 WB,ELISA,IP。

规格价格库存数量
100 μL
¥ 2,279
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产品介绍

生物活性
产品描述
Anti-ETHE1 Antibody (6K797) is a Rabbit antibody targeting ETHE1. Anti-ETHE1 Antibody (6K797) can be used in WB,ELISA,IP.
别名YF13H12, HSCO, ethylmalonic encephalopathy 1
Ig Type
Rabbit IgG
克隆号
6K797
交叉反应
Human
验证活性
1. Anti-ETHE1 rabbit monoclonal antibody at 1:500 dilution.
-Lane A: HepG2 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:28 kDa.
-Observed band size:28 kDa.
2. ETHE1 was immunoprecipitated using:
-Lane A:0.5 mg Hela Whole Cell Lysate.
-Lane B:0.5 mg Jurkat Whole Cell Lysate.
-2 µL anti-ETHE1 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-ETHE1 rabbit monoclonal antibody, at 1:100 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 28 kDa.
-Observed band size: 28 kDa
应用
WB,ELISA,IP
推荐剂量
WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human ETHE1 / HSCO (rh ETHE1 / HSCO; TMPY-03658; O95571; Leu13-Ala254).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景ETHE1, also known as HSCO, is a sulfur dioxygenase that localizes within the mitochondrial matrix. ETHE1 probably plays an important role in metabolic homeostasis in mitochondria. It may also function as a nuclear-cytoplasmic shuttling protein that binds transcription factor RELA/NFKB3 in the nucleus and exports it to the cytoplasm. ETHE1 can suppresses p53-induced apoptosis by preventing nuclear localization of RELA. Mutations in ETHE1 gene result in ethylmalonic encephalopathy. Ethylmalonic encephalopathy is an autosomal recessive, invariably fatal disorder characterized by early-onset encephalopathy, microangiopathy, chronic diarrhea, defective cytochrome c oxidase (COX) in muscle and brain, high concentrations of C4 and C5 acylcarnitines in blood and high excretion of ethylmalonic acid in urine.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: Human ETHE1 / HSCO Protein (TMPY-03658)
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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