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Anti-EphB4 Antibody (6A752) 是一种 Mouse 抗体,靶向 EphB4。Anti-EphB4 Antibody (6A752) 可用于 ELISA,FCM,ICC/IF。
Anti-EphB4 Antibody (6A752) 是一种 Mouse 抗体,靶向 EphB4。Anti-EphB4 Antibody (6A752) 可用于 ELISA,FCM,ICC/IF。
产品描述 | Anti-EphB4 Antibody (6A752) is a Mouse antibody targeting EphB4. Anti-EphB4 Antibody (6A752) can be used in ELISA,FCM,ICC/IF. |
Ig Type | Monoclonal Mouse IgG1 |
克隆号 | 6A752 |
交叉反应 | Human |
验证活性 | 1. Flow cytometric analysis of human EphB4 expression on MCF-7 cells. Cells were stained with purified anti-Human EphB4, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells. 2. Immunofluorescence staining of EPhB4 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human EPhB4 monoclonal antibody (1:30) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green) and counterstained with DAPI(blue). |
应用 | ELISA,FCM,ICC/IF |
推荐剂量 | ELISA: 1:1000-1:2000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EphB4 / HTK extracellular domain (rhEphB4; TMPY-00787; NP_004435.3; Met 1-Ala 539). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
纯化方式 | Protein A |
性状 | Liquid |
缓冲液 | 0.2 μm filtered solution in PBS |
研究背景 | Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore, EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Therefore, Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers. |
偶联 | Unconjugated |
其他可选修饰符 | APC/FITC/PE |
可提供抗体种类 | 4 |
免疫原 | Recombinant Human EphB4 protein (TMPY-00787) |
抗原种属 | Human |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
运输方式 | Shipping with blue ice. |
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