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Anti-ENTPD5 Antibody (2A699)
还可以产品编号 TMAY-01725
Anti-ENTPD5 Antibody (2A699) 是一种 Rabbit 抗体，靶向 ENTPD5。Anti-ENTPD5 Antibody (2A699) 可用于 WB,ELISA(Cap),IHC-P,IP。
Anti-ENTPD5 Antibody (2A699)
Anti-ENTPD5 Antibody (2A699)
还可以产品编号 TMAY-01725
Anti-ENTPD5 Antibody (2A699) 是一种 Rabbit 抗体，靶向 ENTPD5。Anti-ENTPD5 Antibody (2A699) 可用于 WB,ELISA(Cap),IHC-P,IP。
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抗原信息
| 产品描述 | Anti-ENTPD5 Antibody (2A699) is a Rabbit antibody targeting ENTPD5. Anti-ENTPD5 Antibody (2A699) can be used in WB,ELISA(Cap),IHC-P,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 2A699 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunochemical staining of human ENTPD5 in human prostate with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections. 2. Immunochemical staining of human ENTPD5 in human liver with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections. 3. Immunochemical staining of human ENTPD5 in human kidney with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections. 4. Anti-ENTPD5 rabbit monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:47 kDa. -Observed band size:53 kDa. 5. ENTPD5 was immunoprecipitated using: -Lane A:0.5 mg HepG2 Whole Cell Lysate -0.5 µL anti-ENTPD5 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-ENTPD5 rabbit monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution. -Developed using the DAB staining technique. -Performed under reducing conditions. -Predicted band size: 47 kDa. -Observed band size: 47 kDa |
| 应用 | WB,ELISA(Cap),IHC-P,IP |
| 推荐剂量 | WB: 1:500-1:1000; IHC-P: 1:500-1:2000; IP: 0.2-1 μL/mg of lysate; ELISA(Cap): 1:1000-1:10000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human ENTPD5 (rh ENTPD5; TMPY-02758; O75356; Met1-His428). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Ectonucleoside triphosphate diphosphohydrolase 5 (ENTPD5), also known as CD39 antigen-like 4, ER-UDPase, Guanosine-diphosphatase ENTPD5, Nucleoside diphosphatase Uridine-diphosphatase ENTPD5. This hydrolase is expressed in response to phosphoinositide 3-kinase (PI3K) signaling. Activation of PI3K results in FOXO phosphorylation by AKT1 and loss of ENTPD5 transcriptional repression. It is Up-regulated in PTEN-deficient cells. Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation.ENTPD5 promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, ENTPD5 constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. ENTPD5 also hydrolyzes GDP and IDP but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. This enzyme Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human ENTPD5 protein (TMPY-02758) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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