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Anti-ENO3 Antibody (6E160)
还可以产品编号 TMAY-02709
Anti-ENO3 Antibody (6E160) 是一种 Rabbit 抗体，靶向 ENO3。Anti-ENO3 Antibody (6E160) 可用于 WB,IHC-P,IP。
Anti-ENO3 Antibody (6E160)
Anti-ENO3 Antibody (6E160)
还可以产品编号 TMAY-02709
Anti-ENO3 Antibody (6E160) 是一种 Rabbit 抗体，靶向 ENO3。Anti-ENO3 Antibody (6E160) 可用于 WB,IHC-P,IP。
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产品介绍
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偶联与修饰
抗原信息
| 产品描述 | Anti-ENO3 Antibody (6E160) is a Rabbit antibody targeting ENO3. Anti-ENO3 Antibody (6E160) can be used in WB,IHC-P,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 6E160 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunochemical staining of human ENO3 in human skeletal muscle with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). 2. Anti-ENO3 rabbit monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell Lysate. -Lane B: Raji Whole Cell Lysate. -Lane C: Hela Whole Cell Lysate. -Lane D: MOLT-4 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:47 kDa. -Observed band size:50 kDa. 3. ENO3 was immunoprecipitated using: -Lane A:0.5 mg Raji Whole Cell Lysate. -2 µL anti-ENO3 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-ENO3 rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Clean-Blotô IP Detection Reagent (HRP) at 1:500 dilution. -Developed using the DAB staining technique. -Performed under reducing conditions. -Predicted band size: 47 kDa. -Observed band size: 53 kDa |
| 应用 | WB,IHC-P,IP |
| 推荐剂量 | WB: 1:500-1:2000; IHC-P: 1:100-1:500; IP: 1-4 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human ENO3 / beta-enolase (rh ENO3 / beta-enolase; TMPY-03446; AAH17249.1; Met1-Lys434). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | ENO3 is one of the three enolase isoenzymes found in mammals. As a homodimer, ENO3 is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in ENO3 gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Two transcripts have been identified for ENO3 gene that differ only in their 5' UTR. ENO3 may play a role in muscle development and regeneration. It appears to have a function in striated muscle development and regeneration. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human ENO3 / beta-enolase Protein (TMPY-03446) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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