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Anti-Emi1 Antibody (9H429)
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产品编号 TMAC-01288
别名 fbxo5, Fbxo31, FBX5_HUMAN, FBX5, F-box only protein 5, F box protein Fbx5, F box protein Fbx 5, F box protein 5, F box only protein 5, EMI1, Emi 1, Early mitotic inhibitor 1
Anti-Emi1 Antibody (9H429) 是一种 Rabbit 抗体，靶向 Emi1。Anti-Emi1 Antibody (9H429) 可用于 WB,ICC,IHC。
Anti-Emi1 Antibody (9H429)
Anti-Emi1 Antibody (9H429)
还可以产品编号 TMAC-01288 别名 fbxo5, Fbxo31, FBX5_HUMAN, FBX5, F-box only protein 5, F box protein Fbx5, F box protein Fbx 5, F box protein 5, F box only protein 5, EMI1, Emi 1, Early mitotic inhibitor 1
Anti-Emi1 Antibody (9H429) 是一种 Rabbit 抗体，靶向 Emi1。Anti-Emi1 Antibody (9H429) 可用于 WB,ICC,IHC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,480 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-Emi1 Antibody (9H429) is a Rabbit antibody targeting Emi1. Anti-Emi1 Antibody (9H429) can be used in WB,ICC,IHC. |
| 别名 | fbxo5, Fbxo31, FBX5_HUMAN, FBX5, F-box only protein 5, F box protein Fbx5, F box protein Fbx 5, F box protein 5, F box only protein 5, EMI1, Emi 1, Early mitotic inhibitor 1 |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Emi1 antibody. Counter stained with hematoxylin. 2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Emi1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-Emi1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Emi1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Emi1 antibody. Counter stained with hematoxylin. 6. ICC staining Emi1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Emi1 in Siha cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| 应用 | |
| 推荐剂量 | IHC: 1:50-200; ICC: 1:50-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Emi1 (for early mitotic inhibitor) regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry. Emi1 is required to arrest unfertilized eggs at metaphase of meiosis II and may be the long-sought mediator of CSF activity. Human Emi1 is similar to Xenopus laevis Emi1, which inhibits the APC (Cdc20) ubiquitination complex to allow accumulation of cyclin B. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein: human Emi1 aa 50-250 |
| 抗原种属 | human |
| Uniprot ID |
| 分子量 | Theoretical: 50 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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