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Anti-EIF2S1 Polyclonal Antibody
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产品编号 TMAB-00601
Anti-EIF2S1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 EIF2S1。Anti-EIF2S1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-EIF2S1 Polyclonal Antibody
Anti-EIF2S1 Polyclonal Antibody
还可以产品编号 TMAB-00601
Anti-EIF2S1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 EIF2S1。Anti-EIF2S1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,160 | 5日内发货 | |
| 100 μL | ¥ 1,975 | 5日内发货 | |
| 200 μL | ¥ 2,780 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-EIF2S1 Polyclonal Antibody is a Rabbit antibody targeting EIF2S1. Anti-EIF2S1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Human,Rat (predicted:Mouse,Chicken,Cow,Rabbit,Pig) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (rat lung tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (eIF2 alpha) Polyclonal Antibody, Unconjugated (TMAB-00601) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAb staining. 2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (EIF2S1/eIF2 alpha) Polyclonal Antibody, Unconjugated (TMAB-00601) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Sample: Lane 1: A431 (Human) Cell Lysate at 30 μg Lane 2: Hela (Human) Cell Lysate at 30 μg Lane 3: Jurkat (Human) Cell Lysate at 30 μg Primary: Anti-EIF2S1/eIF2 alpha (TMAB-00601) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36-38 kDa Observed band size: 35 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:200-800 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cytoplasmic granule. Note=The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Co-localizes with NANOS3 in the stress granules. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | eIF2 alpha is a 36 kDa protein which is ubiquitously expressed in many cell types. The eIF2 protein, which is composed of three subunits (alpha, beta and gamma), is one of the key molecules in the initiation of translation. In mammalian cells, eIF2 alpha is phosphorylated at serine 51 (human EIF2 alpha, the equivalent residue in mouse is serine 52) by at least two kinases: the haem-controlled repressor (HCR) and the interferon inducible double stranded RNA-dependent protein kinase (PKR). Phosphorylation of eIF2 alpha blocks the GDP-GTP exchange activity of eIF2 beta, resulting in the suppression of protein synthesis. The phosphorylation of eIF2 alpha is an important regulatory process in protein synthesis. |
| 免疫原 | KLH conjugated synthetic peptide: human eIF2 alpha |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | RNAi,Regulation,Eukaryotic Initiation factors (eIF's) |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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