Anti-DNMT1 Antibody (2S499) is an antibody targeting DNMT1. Anti-DNMT1 Antibody (2S499) can be used in ELISA, IHC, IF.

Rabbit IgG

2S499

Human

1. IHC image of TMAH-00360 diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
2. Immunofluorescence staining of HepG2 Cells with TMAH-00360 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

ELISA, IHC, IF

IHC:1:50-1:200; IF:1:20-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human Dnmt1

Human

1786

Epigenetics and Nuclear Signaling