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Anti-DDB1 Antibody (1Y859) 是一种 Rabbit 抗体,靶向 DDB1。Anti-DDB1 Antibody (1Y859) 可用于 WB,ICC/IF,IHC,IP。
Anti-DDB1 Antibody (1Y859) 是一种 Rabbit 抗体,靶向 DDB1。Anti-DDB1 Antibody (1Y859) 可用于 WB,ICC/IF,IHC,IP。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,495 | 5日内发货 | |
100 μL | ¥ 2,495 | 5日内发货 |
产品描述 | Anti-DDB1 Antibody (1Y859) is a Rabbit antibody targeting DDB1. Anti-DDB1 Antibody (1Y859) can be used in WB,ICC/IF,IHC,IP. |
别名 | XPE-binding factor, XPE-BF, XPE binding factor, XPE BF, XPE, XPCe, Xeroderma pigmentosum group E-complementing protein, Xeroderma pigmentosum group E complementing protein, XAP-1, XAP1, XAP 1, X associated protein 1, UV-DDB 1, UV-damaged DNA-binding protein 1, UV-damaged DNA-binding factor, UV DDB1, UV DDB 1, UV damaged DNA binding protein 1, UV damaged DNA binding factor, HBV X-associated protein 1, DNA damage-binding protein a, DNA damage-binding protein 1, DNA damage binding protein 1, DDBa, DDB1_HUMAN, Ddb1, DDB p127 subunit, DDB 1, Damage-specific DNA-binding protein 1, Damage specific DNA binding protein 1 |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of DDB1 on different lysates using anti-DDB1 antibody at 1/500 dilution. Positive control: Lane 1: HepG2, Lane 2: NIH-3T3, Lane 3: MCF-7, Lane 4: Rat kidney tissue. 2. Immunohistochemical analysis of paraffin-embedded rat esophagus tissue using anti-DDB1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-DDB1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DDB1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DDB1 antibody. Counter stained with hematoxylin. 6. ICC staining DDB1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining DDB1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining DDB1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC: 1:50-200; ICC/IF: 1:50-200; IP: 1:10-50 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Damaged DNA binding protein (DDB) is a heterodimer composed of two subunits, p127 and p48, which are designated DDB1 and DDB2, respectively. The DDB heterodimer is involved in repairing DNA damaged by ultraviolet light. Specifically, DDB, also designated UV-damaged DNA binding protein (UV-DDB), xeroderma pigmentosum group E binding factor (XPE-BF) and hepatitis B virus X-associated protein 1 (XAP-1), binds to damaged cyclobutane pyrimidine dimers (CPDs). Mutations in the DDB2 gene are implicated as causes of xeroderma pigmentosum group E, an autosomal recessive disease in which patients are defective in nucleotide excision DNA repair. XPE is characterized by hypersensitivity of the skin to sunlight with a high frequency of skin cancer as well as neurologic abnormalities. The hepatitis B virus (HBV) X protein interacts with DDB1, which may mediate HBx transactivation. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 127 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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