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顶部生物活性储存和溶解信息计算器

Anti-CPLX2 Antibody (3Z959)

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Anti-CPLX2 Antibody (3Z959)
产品编号 TMAY-02146
别名 Hfb1, CPX-2, CPX2, complexin 2, 921-L

Anti-CPLX2 Antibody (3Z959) 是一种 Rabbit 抗体，靶向 CPLX2。Anti-CPLX2 Antibody (3Z959) 可用于 FCM,ICC/IF。

Anti-CPLX2 Antibody (3Z959)

Anti-CPLX2 Antibody (3Z959)

Rating icon 还可以
Anti-CPLX2 Antibody (3Z959)
产品编号 TMAY-02146 别名 Hfb1, CPX-2, CPX2, complexin 2, 921-L

Anti-CPLX2 Antibody (3Z959) 是一种 Rabbit 抗体，靶向 CPLX2。Anti-CPLX2 Antibody (3Z959) 可用于 FCM,ICC/IF。

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100 μL
¥ 2,284
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产品介绍

生物活性
产品描述
Anti-CPLX2 Antibody (3Z959) is a Rabbit antibody targeting CPLX2. Anti-CPLX2 Antibody (3Z959) can be used in FCM,ICC/IF.
别名Hfb1, CPX-2, CPX2, complexin 2, 921-L
Ig Type
Rabbit IgG
克隆号
3Z959
交叉反应
Human
验证活性
1. Immunofluorescence staining of CPLX2 in SHSY5Y cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human CPLX2 monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).
2. Flow cytometric analysis of Human CPLX2 expression on SHSY5Y cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human CPLX2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
应用
FCM
ICC/IF
推荐剂量
ICC-IF: 1:20-1:100; FCM: 1:25-1:100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human Complexin-2 / CPLX2 (rh Complexin-2 / CPLX2; TMPY-02025; Q6PUV4-1; Asp2-Lys134).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景Complexin-2 (CPLX2), a member of the complexin/synaphin family, is a soluble pre-synaptic protein believed to regulate neurotransmitter release from pre-synaptic terminals. Complexins are soluble proteins that regulate the activity of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes necessary for vesicle fusion. Complexins are unable to bind to monomeric SNARE proteins but bind with high affinity to ternary SNARE complexes and with lower affinity to target SNARE complexes. Complexin 1 (CX1) and complexin 2 (CX2) are presynaptic proteins that modulate neurotransmitter release and are used as markers of inhibitory and excitatory synapses, respectively. CPLX2 is localized in pre-synaptic terminals in mature brain. The G71-P89 region of CPLX2 is essential and sufficient for preferential axonal distribution. CPLX2 participates in the Ca(2+)-sensitive regulatory pathway for zymogen granule exocytosis. Complexin-2 is a key player in normal neurological function, and its downregulation could lead to changes in neurotransmitter release sufficient to cause significant behavioural abnormalities such as depression. It is involved in synaptogenesis and the modulation of neurotransmitter release.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: Human Complexin-2 / CPLX2 Protein (TMPY-02025)
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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CPX 2

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