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Anti-Chorionic Gonadotropin/CGA Antibody (3J474) 是一种 Mouse 抗体,靶向 Chorionic Gonadotropin/CGA。Anti-Chorionic Gonadotropin/CGA Antibody (3J474) 可用于 IHC-P,IHC-Fr,IF,ELISA。
Anti-Chorionic Gonadotropin/CGA Antibody (3J474) 是一种 Mouse 抗体,靶向 Chorionic Gonadotropin/CGA。Anti-Chorionic Gonadotropin/CGA Antibody (3J474) 可用于 IHC-P,IHC-Fr,IF,ELISA。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,170 | 5日内发货 | |
100 μL | ¥ 1,960 | 5日内发货 | |
200 μL | ¥ 2,790 | 5日内发货 |
产品描述 | Anti-Chorionic Gonadotropin/CGA Antibody (3J474) is a Mouse antibody targeting Chorionic Gonadotropin/CGA. Anti-Chorionic Gonadotropin/CGA Antibody (3J474) can be used in IHC-P,IHC-Fr,IF,ELISA. |
Ig Type | IgG |
克隆号 | 3J474 |
交叉反应 | Human |
验证活性 | 1. Measured by its binding ability in a indirect ELISA. Immobilized Human HCG alpha protein, HisTag at 2 μg/mL (100 μL/well) can bind Mouse Anti-Human HCG alpha Antibody, the EC50 is 40.93 ng/mL. 2. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CGA Monoclonal Antibody, Unconjugated (TMAB-00428) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human pituitary gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CGA Monoclonal Antibody, Unconjugated (TMAB-00428) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 4. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CGA Monoclonal Antibody, Unconjugated (TMAB-00428) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. |
应用 | |
推荐剂量 | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000 |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
亚细胞定位 | Secreted. |
构建方式 | Hybridoma Monoclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M PBS (pH7.4) with 0.02% Proclin300. |
研究背景 | The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] |
免疫原 | Natural human hCG protein |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Hormone biosynthesis,Hormone biosynthesis,Cancer,Gonadotrophic axis,Thyroid axis,Hormones |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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