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Anti-CD68 Antibody (8F211)

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Anti-CD68 Antibody (8F211)
产品编号 TMAB-00380

Anti-CD68 Antibody (8F211) 是一种 Mouse 抗体,靶向 CD68。Anti-CD68 Antibody (8F211) 可用于 IHC-P,IHC-Fr,IF。

Anti-CD68 Antibody (8F211)

Anti-CD68 Antibody (8F211)

Rating icon 还可以
Anti-CD68 Antibody (8F211)
产品编号 TMAB-00380

Anti-CD68 Antibody (8F211) 是一种 Mouse 抗体,靶向 CD68。Anti-CD68 Antibody (8F211) 可用于 IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,160
5日内发货
100 μL
¥ 1,975
5日内发货
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产品介绍

生物活性
产品描述
Anti-CD68 Antibody (8F211) is a Mouse antibody targeting CD68. Anti-CD68 Antibody (8F211) can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
克隆号
8F211
交叉反应
Human,Rat
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD68) Monoclonal Antibody, Unconjugated (TMAB-00380) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD68) Monoclonal Antibody, Unconjugated (TMAB-00380) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD68) Monoclonal Antibody, Unconjugated (TMAB-00380) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (Human diffuse large B-cell lymphoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD68) Monoclonal Antibody, Unconjugated (TMAB-00380) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD68) Monoclonal Antibody, Unconjugated (TMAB-00380) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD68) Monoclonal Antibody, Unconjugated (TMAB-00380) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
应用
推荐剂量
IHC-P: 1:100-300; IHC-Fr: 1:100-300; IF: 1:100-300
抗体种类
Monoclonal
宿主来源Mouse
亚细胞定位Isoform Short: Cell membrane; Single-pass type I membrane protein. Isoform Long: Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein.
构建方式Hybridoma Monoclonal Antibody
纯化方式Protein G purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]
抗原信息
免疫原
Recombinant Protein: human CD68 1-100/354aa
抗原种属
Human
基因ID
Uniprot ID
研究领域
Myeloid Cells,SARS Coronavirus
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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