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Anti-CD3D Antibody (5P835)
还可以产品编号 TMAY-01417
Anti-CD3D Antibody (5P835) 是一种 Rabbit 抗体，靶向 CD3D。Anti-CD3D Antibody (5P835) 可用于 WB,IP。
Anti-CD3D Antibody (5P835)
Anti-CD3D Antibody (5P835)
还可以产品编号 TMAY-01417
Anti-CD3D Antibody (5P835) 是一种 Rabbit 抗体，靶向 CD3D。Anti-CD3D Antibody (5P835) 可用于 WB,IP。
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抗原信息
| 产品描述 | Anti-CD3D Antibody (5P835) is a Rabbit antibody targeting CD3D. Anti-CD3D Antibody (5P835) can be used in WB,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 5P835 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-CD3D rabbit monoclonal antibody at 1:500 dilution. -Lane A: Jurkat Whole Cell Lysate. -Lane B: Hu-T78 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:19 kDa. -Observed band size:24 kDa. 2. CD3D was immunoprecipitated using: -Lane A:0.5 mg Jurkat Whole Cell Lysate. -Lane B:0.5 mg HuT78 Whole Cell Lysate. -Lane C:0.5 mg Caco-2 Whole Cell Lysate -0.5 µL anti-CD3D rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-CD3D rabbit monoclonal antibody, at 1:250 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 20 kDa. -Observed band size: 20 kDa |
| 应用 | WB,IP |
| 推荐剂量 | WB: 1:500-1:1000; IP: 0.2-1 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human CD3D / CD3 Delta (rh CD3D / CD3 Delta; TMPY-01196; NP_000723.1; Met1-Ala105). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | T-cell surface glycoprotein CD3 delta chain, also known as CD3D, is a single-pass type I membrane protein. CD3D, together with CD3-gamma, CD3-epsilon and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The majority of T cell receptor (TCR) complexes in mice and humans consist of a heterodimer of polymorphic TCR alpha and beta chains along with invariant CD3 gamma, delta, epsilon, and zeta chains. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). CD3D contains 1 ITAM domain and has been shown to interact with CD8A. In the mouse, knockout of CD3delta allows some degree of T lymphocyte differentiation since mature CD4 and CD8 as well as TCRgammadelta T lymphocytes are observed in the periphery. In contrast, deleterious mutation of the CD3 delta encoding gene in the human leads to a severe combined immunodeficiency characterised by the complete absence of mature T cell subpopulations including TCRalpha/beta and TCR gamma/delta. Defects in CD3D cause severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T-/B+/NK+ SCID) which is a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. In humans the absence of CD3 delta results in a complete arrest in thymocyte development at the stage of double negative to double positive transition and the development of gamma delta T-cell receptor-positive T cells is also impaired.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human CD3D / CD3 Delta protein (TMPY-01196) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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